Elp3 Antibody (11811) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43694
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Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 11811
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the center region of human Elp3. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Theoretical MW
62 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.
Scientific Data Images for Elp3 Antibody (11811) - Azide and BSA Free
Western Blot: Elp3 Antibody (11811) [NBP2-43694]
Western Blot: Elp3 Antibody (11811) [NBP2-43694] - Analysis of various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:500.Western Blot: Elp3 Antibody (11811) [NBP2-43694]
Western Blot: Elp3 Antibody (11811) [NBP2-43694] - Analysis of HeLa whole cell extracts and nuclear extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with ELP3 antibody diluted by 1:1000.Immunoprecipitation: Elp3 Antibody (11811) [NBP2-43694]
Immunoprecipitation: Elp3 Antibody (11811) [NBP2-43694] - Analysis of ELP3 protein from 293T whole cell extracts using 5 ug of ELP3 antibody [11811]. Western blot analysis was performed using ELP3 antibody [11811]. EasyBlot anti-Mouse IgG was used as a secondary reagent.Immunocytochemistry/ Immunofluorescence: Elp3 Antibody (11811) [NBP2-43694] -
ELP3 antibody [GT11811] detects ELP3 protein at nucleus by immunofluorescent analysis.Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: ELP3 protein stained by ELP3 antibody [GT11811] (NBP2-43694) diluted at 1:500.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.
Applications for Elp3 Antibody (11811) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:100-1:1000
Immunoprecipitation
1:100-1:500
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: Elp3
Alternate Names
EC 2.3.1.48, elongation protein 3 homolog (S. cerevisiae), elongator complex protein 3, hELP3, KAT9FLJ10422
Gene Symbol
ELP3
Additional Elp3 Products
Product Documents for Elp3 Antibody (11811) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for Elp3 Antibody (11811) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Immunoprecipitation Protocol
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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