EZH2/KMT6 Antibody - BSA Free

Novus Biologicals | Catalog # NBP2-17087

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Rat

Predicted:

Bovine (99%), Fish (95%), Mouse (99%), Porcine (99%), Rhesus Macaque (100%), Xenopus (97%), Zebrafish (94%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Chromatin Immunoprecipitation (ChIP)

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all EZH2 Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human EZH2/KMT6. The exact sequence is proprietary.

Reactivity Notes

Xenopus laevis (97%), Japanese medaka (95%),

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

85 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for EZH2/KMT6 Antibody - BSA Free

Western Blot: EZH2/KMT6 Antibody [NBP2-17087]

Western Blot: EZH2/KMT6 Antibody [NBP2-17087]

Western Blot: KMT6/EZH2 Antibody [NBP2-17087] - Sample (30 ug of whole cell lysate) A: H1299 B: HCT116 C: MCF-7 7. 5% SDS PAGE gel, diluted at 1:1000.
EZH2/KMT6 Antibody - BSA Free

Western Blot: EZH2/KMT6 Antibody [NBP2-17087] -

Various whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with EZH2 antibody (NBP2-17087) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
EZH2/KMT6 Antibody - BSA Free

Chromatin Immunoprecipitation: EZH2/KMT6 Antibody [NBP2-17087] -

Cross-linked ChIP was performed with HeLa chromatin extract and 5 ug of either control rabbit IgG or anti-EZH2 antibody. The precipitated DNA was detected by PCR with primer set targeting to CCND2.
EZH2/KMT6 Antibody - BSA Free

Immunocytochemistry/ Immunofluorescence: EZH2/KMT6 Antibody [NBP2-17087] -

EZH2 antibody detects EZH2 protein at nucleus by immunofluorescent analysis.
Sample: HeLa cells were fixed in 4% paraformaldehyde at RT for 15 min.
Green: EZH2 protein stained by EZH2 antibody (NBP2-17087) diluted at 1:500.
Red: phalloidin, a cytoskeleton marker, diluted at 1:200.
Blue: Hoechst 33342 staining.
Scale bar = 10 um.

Applications for EZH2/KMT6 Antibody - BSA Free

Application
Recommended Usage

Chromatin Immunoprecipitation (ChIP)

Assay dependent

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 20% Glycerol

Format

BSA Free

Preservative

0.025% Proclin 300

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: EZH2

This gene encodes a member of the Polycomb-group (PcG) family. PcG family members form multimeric protein complexes, which are involved in maintaining the transcriptional repressive state of genes over successive cell generations. This protein associates with the embryonic ectoderm development protein, the VAV1 oncoprotein, and the X-linked nuclear protein. This protein may play a role in the hematopoietic and central nervous systems. Two transcript variants encoding distinct isoforms have been identified for this gene. [provided by RefSeq]

Long Name

Enhancer of Zeste Homolog 2 [Drosophila]

Alternate Names

ENX1, KMT6

Gene Symbol

EZH2

UniProt

Q15910

Additional EZH2 Products

Product Documents for EZH2/KMT6 Antibody - BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for EZH2/KMT6 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

Related Research Areas

Cancer Biomarkers
Epigenetics

Citations for EZH2/KMT6 Antibody - BSA Free

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Protocols

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