Fibrillarin Antibody

Novus Biologicals | Catalog # NBP2-16492

Novus Biologicals
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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Fibrillarin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the center region of human Fibrillarin. The exact sequence is proprietary.

Localization

Nucleus; nucleolus

Marker

Nucleolar Marker

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Theoretical MW

34 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Scientific Data Images for Fibrillarin Antibody

Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492] - Fibrillarin antibody immunoprecipitates FBL protein in IP experiments. IP samples: HeLa nuclear extract. A. 35 ug HeLa nuclear extract. B. Control with 4 ug of preimmune Rabbit IgG. C. Immunoprecipitation of FBL protein by 4 ug Fibrillarin antibody. 10 % SDS-PAGE. The immunoprecipitated FBL protein was detected by Fibrillarin antibody diluted at 1:1000.
Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody [NBP2-16492]

Immunocytochemistry/ Immunofluorescence: Fibrillarin Antibody [NBP2-16492]

Immunocytochemistry/Immunofluorescence: Fibrillarin Antibody [NBP2-16492] - Confocal immunofluorescence analysis of paraformaldehyde-fixed HeLa, using Fibrillarin antibody (Green) at 1:500 dilution. Alpha-tubulin filaments are labeled with Alpha-tubulin antibody (Red) at 1:2000.
Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492] - 30 ug Rat whole cell lysate/extract 12 % SDS-PAGE Fibrillarin antibody dilution: 1:1000.
Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A: 293T B: H1299 12% SDS PAGE gel, diluted at 1:1000.
Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492]

Western Blot: Fibrillarin Antibody [NBP2-16492] - Sample (30 ug of whole cell lysate) A:NIH-3T3 10% SDS PAGE gel, diluted at 1:1000.

Applications for Fibrillarin Antibody

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100-1:1000

Immunoprecipitation

1:100-1:500

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Formulation

PBS, 1% BSA, 20% Glycerol

Preservative

0.01% Thimerosal

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.

Background: Fibrillarin

This gene product is a component of a nucleolar small nuclear ribonucleoprotein (snRNP) particle thought to participate in the first step in processing preribosomal RNA. It is associated with the U3, U8, and U13 small nuclear RNAs and is located in the dense fibrillar component (DFC) of the nucleolus. The encoded protein contains an N-terminal repetitive domain that is rich in glycine and arginine residues, like fibrillarins in other species. Its central region resembles an RNA-binding domain and contains an RNP consensus sequence. Antisera from approximately 8% of humans with the autoimmune disease scleroderma recognize fibrillarin. [provided by RefSeq]

Alternate Names

EC 2.1.1,34-kD nucleolar scleroderma antigen, EC 2.1.1.-, EC 2.1.1.37, FIB, FIB1,34 kDa nucleolar scleroderma antigen, fibrillarin, FLRNrRNA 2'-O-methyltransferase fibrillarin, RNA, U3 small nucleolar interacting protein 1, RNU3IP1

Gene Symbol

FBL

Additional Fibrillarin Products

Product Documents for Fibrillarin Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Download SDS

Product Specific Notices for Fibrillarin Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including mercury, which is known to the State of California to cause reproductive toxicity with developmental effects.  For more information go to www.P65Warnings.ca.gov.

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Protocols

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

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