FOXO4 [p Ser197] Antibody - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-05393

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Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

Azide and BSA Free
View all FOXO4 Primary Antibodies »

Product Specifications

Immunogen

A synthetic phosphorylated peptide around S197 of human FOXO4 (NP_005929.2). AASMD

Modification

p Ser197

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for FOXO4 [p Ser197] Antibody - Azide and BSA Free

Western Blot: FOXO4 [p Ser197] AntibodyAzide and BSA Free [NBP3-05393]

Western Blot: FOXO4 [p Ser197] AntibodyAzide and BSA Free [NBP3-05393]

Western Blot: FOXO4 [p Ser197] Antibody [NBP3-05393] - Western blot analysis of extracts from 293 cells using phospho-FOXO4-S197 antibody (NBP3-05393). Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% BSA.
Immunocytochemistry/ Immunofluorescence: FOXO4 [p Ser197] Antibody - Azide and BSA Free [NBP3-05393]

Immunocytochemistry/ Immunofluorescence: FOXO4 [p Ser197] Antibody - Azide and BSA Free [NBP3-05393]

Immunocytochemistry/Immunofluorescence: FOXO4 [p Ser197] Antibody [NBP3-05393] - Immunofluorescence analysis of methanol-fixed MCF-7 cells using FOXO4 antibody (NBP3-05393).
Immunohistochemistry-Paraffin: FOXO4 [p Ser197] Antibody - Azide and BSA Free [NBP3-05393]

Immunohistochemistry-Paraffin: FOXO4 [p Ser197] Antibody - Azide and BSA Free [NBP3-05393]

Immunohistochemistry-Paraffin: FOXO4 [p Ser197] Antibody [NBP3-05393] - Immunohistochemistry of paraffin-embedded human breast carcinoma tissue, using FOXO4 antibody (NBP3-05393).
FOXO4 [p Ser197] Antibody - Azide and BSA Free

Western Blot: FOXO4 [p Ser197] Antibody - Azide and BSA Free [FOXO4] -

Western Blot: FOXO4 [p Ser197] Antibody - Azide and BSA Free [FOXO4] - Western blot analysis of various lysates, using FOXO4 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.
FOXO4 [p Ser197] Antibody - Azide and BSA Free

Western Blot: FOXO4 [p Ser197] Antibody - Azide and BSA Free [FOXO4] -

Western Blot: FOXO4 [p Ser197] Antibody - Azide and BSA Free [FOXO4] - Western blot analysis of various lysates, using FOXO4 Rabbit pAb at 1:400 dilution.
Secondary antibody: HRP-conjugated Goat anti-Rabbit IgG (H+L) at 1:10000 dilution.
Lysates/proteins: 25ug per lane.
Blocking buffer: 3% nonfat dry milk in TBST.
Detection: ECL Basic Kit.
Exposure time: 60s.

Applications for FOXO4 [p Ser197] Antibody - Azide and BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:100 - 1:200

Western Blot

1:100 - 1:500

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol

Format

Azide and BSA Free

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: FOXO4

AFX aka FOXO4 is a forkhead transcription factor involved in the regulation of the insulin signaling pathway. It binds to insulin-response elements (IREs) and can activate transcription of IGFBP1. FOXO4 down-regulates expression of HIF1A and suppresses hypoxia-induced transcriptional activation of HIF1A-modulated genes. It is also involved in negative regulation of the cell cycle.

Alternate Names

AFX, AFX1myeloid/lymphoid or mixed-lineage leukemia (trithorax (Drosophila) homolog);translocated to, 7, Fork head domain transcription factor AFX1, forkhead box O4, forkhead box protein O4, MLLT7MGC120490, myeloid/lymphoid or mixed-lineage leukemia (trithorax homolog, Drosophila);translocated to, 7

Gene Symbol

FOXO4

Additional FOXO4 Products

Product Documents for FOXO4 [p Ser197] Antibody - Azide and BSA Free

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

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Product Specific Notices for FOXO4 [p Ser197] Antibody - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

⚠ WARNING: This product can expose you to chemicals including Methotrexate, which is known to the State of California to cause reproductive toxicity with developmental effects. For more information, go to www.P65Warnings.ca.gov

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Protocols

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