gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Novus Biologicals | Catalog # NBP3-25476

Recombinant Monoclonal Antibody
Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human, Mouse, Rat, Canine, Drosophila, Feline

Predicted:

Bovine (100%), Chimpanzee (100%), Rhesus Macaque (100%), Xenopus (99%), Zebrafish (98%). Backed by our 100% Guarantee.

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # HL1175 expressed in HEK293

Format

Azide and BSA Free
View all gamma Tubulin Primary Antibodies »

Product Specifications

Immunogen

Recombinant protein encompassing a sequence within the N-terminus region of human gamma Tubulin. The exact sequence is proprietary.

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for gamma Tubulin Antibody (HL1175) - Azide and BSA Free

gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] -

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:5000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] -

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Whole cell extract (30 ug) was separated by 7.5% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] -

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:10000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] -

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome and mitochondria by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:2500. Blue: Fluoroshield with DAPI.
gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] -

Immunocytochemistry/Immunofluorescence: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - gamma Tubulin antibody [HL1175] detects gamma Tubulin protein at centrosome by immunofluorescent analysis. Sample: HeLa cells were fixed in ice-cold MeOH for 5 min. Green: gamma Tubulin stained by gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:2500. Red: alpha Tubulin, a cytoskeleton marker, stained by alpha Tubulin antibody [GT114] (NBP2-43837) diluted at 1:1000. Blue: Fluoroshield with DAPI.
gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] -

Western Blot: gamma Tubulin Antibody (HL1175) - Azide and BSA Free [NBP3-25476] - Whole cell extract (30 ug) was separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:3000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.
gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Western Blot: gamma Tubulin Antibody (HL1175) [NBP3-25476] -

Various whole cell extracts (30 ug) were separated by 10% SDS-PAGE, and the membrane was blotted with gamma Tubulin antibody [HL1175] (NBP3-25476) diluted at 1:1000. The HRP-conjugated anti-rabbit IgG antibody was used to detect the primary antibody.

Applications for gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Application
Recommended Usage

Western Blot

1:500-1:3000

Formulation, Preparation, and Storage

Purification

Protein A purified

Formulation

PBS

Format

Azide and BSA Free

Preservative

No Preservative

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: gamma Tubulin

The gamma-tubulin (TUBG1; relative molecular weight about 48 kDa) is a minor member of tubulin family (less that 0.01% of tubulin dimer). The g-tubulin ring structures, however, serve to provide structural primer for initiation of microtubular nucleation and growth, thereby being crutial for microtubule-based cellular processes, above all for mitotic spindle formation. In animal cells, a center of microtubule organization is the centrosome composed of a pair of cylindrical centrioles surrounded by fibrous pericentriolar material containing g-tubulin. Formation of the mitotic spindle is preceded by duplication of centrosome during S phase. Before mitosis, both centrosomes increase their microtubule nucleation capacity and form two microtuble asters that are pushed apart from each other by the forces of motor proteins associated at the microtubule surface.

Long Name

Tubulin gamma-1 chain

Alternate Names

Gamma-1-tubulin, GCP-1, TUBG, TUBG1

Gene Symbol

TUBG1

Additional gamma Tubulin Products

Product Documents for gamma Tubulin Antibody (HL1175) - Azide and BSA Free

Certificate of Analysis

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Product Specific Notices for gamma Tubulin Antibody (HL1175) - Azide and BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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FAQs

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