GEF-H1 Antibody (336) - Azide and BSA Free
Novus Biologicals | Catalog # NBP2-43635
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Key Product Details
Species Reactivity
Human, Mouse, Rat
Applications
Western Blot, Immunocytochemistry/ Immunofluorescence
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 336
Format
Azide and BSA Free
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Product Specifications
Immunogen
Recombinant protein encompassing a sequence within the C-terminus region of human GEF-H1. The exact sequence is proprietary.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Scientific Data Images for GEF-H1 Antibody (336) - Azide and BSA Free
Western Blot: GEF-H1 Antibody (336) [NBP2-43635]
Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Non-transfected (-) and transfected (+) 293T whole cell extracts (30 ug) were separated by 7.5% SDS-PAGE, and the membrane was blotted with GEF-H1 antibody (336) diluted at 1:500. HRP-conjugated anti-mouset IgG antibody was used to detect the primary antibody.Immunocytochemistry/ Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635]
Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: HeLa cells were fixed in 4% paraformaldehyde at room temperature. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50. Blue: Hoechst 33342 staining.Western Blot: GEF-H1 Antibody (336) [NBP2-43635]
Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug 293T whole cell lysate/extract B. 30 ug A431 whole cell lysate/extract C. 30 ug HeLa whole cell lysate/extract 7.5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500Western Blot: GEF-H1 Antibody (336) [NBP2-43635]
Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug C8D30 whole cell lysate/extract B. 30 ug BCL-1 whole cell lysate/extract C. 30 ug Raw264.7 whole cell lysate/extract D. 30 ug C2C12 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500Western Blot: GEF-H1 Antibody (336) [NBP2-43635]
Western Blot: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of A. 30 ug Rat-2 whole cell lysate/extract 5 % SDS-PAGE GEF-H1 antibody [336] dilution: 1:500.Immunocytochemistry/ Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635]
Immunocytochemistry/Immunofluorescence: GEF-H1 Antibody (336) [NBP2-43635] - Analysis of Sample: MDA-MB-231 cells were fixed in methanol for 5 min. Green: GEF-H1 protein stained by GEF-H1 antibody [336] diluted at 1:50.Applications for GEF-H1 Antibody (336) - Azide and BSA Free
Application
Recommended Usage
Immunocytochemistry/ Immunofluorescence
1:50-1:1000
Western Blot
1:500-1:3000
Formulation, Preparation, and Storage
Purification
Protein G purified
Formulation
PBS
Format
Azide and BSA Free
Preservative
No Preservative
Concentration
Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.
Shipping
The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage
Aliquot and store at -20C or -80C. Avoid freeze-thaw cycles.
Background: GEF-H1
Alternate Names
GEF, GEFH1, GEF-H1DKFZp547P1516, Guanine nucleotide exchange factor H1, KIAA0651DKFZp547L106, LFP40FLJ45440, Microtubule-regulated Rho-GEF, P40, Proliferating cell nucleolar antigen p40, rho guanine nucleotide exchange factor 2, Rho/Rac guanine nucleotide exchange factor (GEF) 2, Rho/Rac guanine nucleotide exchange factor 2
Gene Symbol
ARHGEF2
Additional GEF-H1 Products
Product Documents for GEF-H1 Antibody (336) - Azide and BSA Free
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Product Specific Notices for GEF-H1 Antibody (336) - Azide and BSA Free
This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Appropriate Fixation of IHC/ICC Samples
- Cellular Response to Hypoxia Protocols
- ClariTSA™ Fluorophore Kits
- Detection & Visualization of Antibody Binding
- ICC Cell Smear Protocol for Suspension Cells
- ICC Immunocytochemistry Protocol Videos
- ICC for Adherent Cells
- Immunocytochemistry (ICC) Protocol
- Immunocytochemistry Troubleshooting
- Immunofluorescence of Organoids Embedded in Cultrex Basement Membrane Extract
- Immunohistochemistry (IHC) and Immunocytochemistry (ICC) Protocols
- Preparing Samples for IHC/ICC Experiments
- Preventing Non-Specific Staining (Non-Specific Binding)
- Primary Antibody Selection & Optimization
- Protocol for VisUCyte™ HRP Polymer Detection Reagent
- Protocol for the Fluorescent ICC Staining of Cell Smears - Graphic
- Protocol for the Fluorescent ICC Staining of Cultured Cells on Coverslips - Graphic
- Protocol for the Preparation and Fluorescent ICC Staining of Cells on Coverslips
- Protocol for the Preparation and Fluorescent ICC Staining of Non-adherent Cells
- Protocol for the Preparation and Fluorescent ICC Staining of Stem Cells on Coverslips
- Protocol for the Preparation of a Cell Smear for Non-adherent Cell ICC - Graphic
- R&D Systems Quality Control Western Blot Protocol
- TUNEL and Active Caspase-3 Detection by IHC/ICC Protocol
- The Importance of IHC/ICC Controls
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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