Glucagon Antibody (53) - BSA Free

Novus Biologicals | Catalog # NBP1-05184

Novus Biologicals
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Key Product Details

Species Reactivity

Mammal

Applications

Western Blot, ELISA

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 kappa Clone # 53

Format

BSA Free
View all Glucagon Primary Antibodies »

Product Specifications

Immunogen

Synthetic GLP-1(9-18)

Epitope

The free N-terminus of GLP-1(9-36)amide and GLP-1(9-37)

Specificity

Based on inhibition ELISA, It cross-reacts <2% with GLP-1(7-36)amide

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1 kappa

Description

Concentration for any given batch will be between 0.85 - 1.15 mg/mL.

Scientific Data Images for Glucagon Antibody (53) - BSA Free

Glucagon Antibody (53) - BSA Free [NBP1-05184] - BSA Free [NBP1-05184]

Applications for Glucagon Antibody (53) - BSA Free

Application
Recommended Usage

ELISA

1:6000

Western Blot

1:100-1:2000
Application Notes
ELISA: NBP1-05184 binds free GLP-1(9-36)amide in solution. NBP1-05184 has higher affinity for GLP-1(9-36)amide than NBP2-23591 as determined by inhibition. WB: NBP1-05184 can be used in Western blotting.

Formulation, Preparation, and Storage

Purification

Protein A or G purified

Formulation

10mM Phosphate (pH 7.4) and 0.5M NaCl

Format

BSA Free

Preservative

0.09% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C in the dark.

Background: Glucagon

Glucagon-like peptide-1(9-36)amide and GLP-1(9-37) are the forms of GLP-1 that result from the rapid degradation of the active forms of the peptide (GLP-1(7-36)amide and GLP-1(7-37)) by the enzyme dipeptidyl peptidase-IV (DPP-IV, also known as CD26 or adenosine deaminase binding protein). GLP-1 is a peptide hormone of the glucagon family, produced by the L cells of the intestinal mucosa from the same prohormone as glucagon. The active forms are potent stimulators of glucose dependent insulin secretion. The sequence of GLP-1 is fully conserved in all mammalian species examined so far.

Alternate Names

GCG, GRPP

Gene Symbol

GCG

Additional Glucagon Products

Product Documents for Glucagon Antibody (53) - BSA Free

Certificate of Analysis

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Product Specific Notices for Glucagon Antibody (53) - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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