HDAC6 Antibody

Novus Biologicals | Catalog # NB100-61065

Novus Biologicals
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Key Product Details

Validated by

Biological Validation

Species Reactivity

Human, Mouse

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG
View all Histone Deacetylase 6/HDAC6 Primary Antibodies »

Product Specifications

Immunogen

The immunogen recognized by this antibody maps to a region between residue 1165 and 1215 of human histone deacetylase 6 using the numbering given in entry NP_006035.2 (GeneID 10013).

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HDAC6 Antibody

Western Blot: HDAC6 Antibody [NB100-61065]

Western Blot: HDAC6 Antibody [NB100-61065]

Western Blot: HDAC6 Antibody [NB100-61065] - Detection of Human HDAC6 by Western Blot. Samples: Whole cell lysate (50 ug) from HeLa and 293T cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Western Blot: HDAC6 Antibody [NB100-61065]

Western Blot: HDAC6 Antibody [NB100-61065]

Western Blot: HDAC6 Antibody [NB100-61065] - Whole cell lysate (50 ug) from TCMK-1 and NIH3T3 cells prepared using NETN lysis buffer. Antibody: Affinity purified rabbit anti-HDAC6 antibody used for WB at 0.1 ug/ml. Detection: Chemiluminescence with an exposure time of 3 minutes.
Immunoprecipitation: HDAC6 Antibody [NB100-61065]

Immunoprecipitation: HDAC6 Antibody [NB100-61065]

Immunoprecipitation: HDAC6 Antibody [NB100-61065] - Detection of human HDAC6 by western blot of immunoprecipitates. Samples: Whole cell lysate (0.5 or 1.0 mg per IP reaction; 20% of IP loaded) from HeLa cells prepared using NETN lysis buffer. Antibodies: Affinity purified rabbit anti-HDAC6 antibody NB100-61065 (lot NB100-61065-2) used for IP at 6 ug per reaction. HDAC6 was also immunoprecipitated by a previous lot of this antibody NB100-61065-1. For blotting immunoprecipitated HDAC6,NB100-61065 was used at 1 ug/ml. Detection: Chemiluminescence with an exposure time of 30 seconds.

Applications for HDAC6 Antibody

Application
Recommended Usage

Immunoprecipitation

2 - 10 ug/mg lysate

Western Blot

1:2000-1:10000

Formulation, Preparation, and Storage

Purification

Immunogen affinity purified

Formulation

TBS and 0.1% BSA

Preservative

0.09% Sodium Azide

Concentration

0.2 mg/ml

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C. Do not freeze.

Background: Histone Deacetylase 6/HDAC6

Histones play a critical role in transcriptional regulation, cell cycle progression, and developmental events. Histone acetylation/deacetylation alters chromosome structure and affects transcription factor access to DNA. The protein encoded by this gene belongs to class II of the histone deacetylase/acuc/apha family. It contains an internal duplication of two catalytic domains which appear to function independently of each other. This protein possesses histone deacetylase activity and represses transcription.

Long Name

Histone Deacetylase 6

Alternate Names

CPBHM, FLJ16239, HD6, HDAC6, JM21, PPP1R90

Entrez Gene IDs

10013 (Human)

Gene Symbol

HDAC6

UniProt

NP_006035.2 (Human)

Additional Histone Deacetylase 6/HDAC6 Products

Product Documents for HDAC6 Antibody

Certificate of Analysis

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Product Specific Notices for HDAC6 Antibody

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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