Recombinant Human HGF His Protein

Novus Biologicals | Catalog # NBP2-51730

Novus Biologicals
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Key Product Details

Source

E. coli

Tag

His

Applications

SDS-PAGE
View all HGF Proteins and Enzymes »

Product Specifications

Description

A denatured recombinant protein with a His-tag corresponding to the amino acids 32-285 of Human HGF

Source: E.coli

Amino Acid Sequence: MQRKRRNTIH EFKKSAKTTL IKIDPALKIK TKKVNTADQC ANRCTRNKGL PFTCKAFVFD KARKQCLWFP FNSMSSGVKK EFGHEFDLYE NKDYIRNCII GKGRSYKGTV SITKSGIKCQ PWSSMIPHEH SYRGKDLQEN YCRNPRGEEG GPWCFTSNPE VRYEVCDIPQ CSEVECMTCN GESYRGLMDH TESGKICQRW DHQTPHRHKF LPERYPDKGF DDNYCRNPDG QPRPWCYTLD PHTRWEYCAI KTCET

Purity

>80%, by SDS-PAGE

Predicted Molecular Mass

29.8 kDa.
Disclaimer note: The observed molecular weight of the protein may vary from the listed predicted molecular weight due to post translational modifications, post translation cleavages, relative charges, and other experimental factors.

Application Notes

Denatured protein is most likely not the best option for functional studies. It is better suited for Western Blot (WB) or imaging assays.

Protein / Peptide Type

Recombinant Protein

Scientific Data Images for Recombinant Human HGF His Protein

SDS-PAGE: Recombinant Human HGF His Protein [NBP2-51730]

SDS-PAGE: Recombinant Human HGF His Protein [NBP2-51730]

SDS-Page: Recombinant Human HGF Protein [NBP2-51730] - 15% SDS Page (3 ug)

Formulation, Preparation, and Storage

NBP2-51730
Formulation 20 mM Tris-HCl buffer (pH 8.0), 10% glycerol, 0.4M UREA
Preservative No Preservative
Concentration 1 mg/ml
Shipping The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.
Stability & Storage Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HGF

Hepatocyte growth factor regulates cell growth, cell motility, and morphogenesis by activating a tyrosine kinase signaling cascade after binding to the proto-oncogenic c-Met receptor. Hepatocyte growth factor is secreted by mesenchymal cells and acts as a multi-functional cytokine on cells of mainly epithelial origin. Its ability to stimulate mitogenesis, cell motility, and matrix invasion gives it a central role in angiogenesis, tumorogenesis, and tissue regeneration. It is secreted as a single inactive polypeptide and is cleaved by serine proteases into a 69-kDa alpha-chain and 34-kDa beta-chain. A disulfide bond between the alpha and beta chains produces the active, heterodimeric molecule. The protein belongs to the plasminogen subfamily of S1 peptidases but has no detectable protease activity. Alternative splicing of this gene produces multiple transcript variants encoding different isoforms. [provided by RefSeq]

Long Name

Hepatocyte Growth Factor

Alternate Names

DFNB39, F-TCF, Hepatopoietin A, HGFB, HPTA, SF

Gene Symbol

HGF

Additional HGF Products

Product Documents for Recombinant Human HGF His Protein

Certificate of Analysis

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Product Specific Notices for Recombinant Human HGF His Protein

This product is for research use only and is not approved for use in humans or in clinical diagnosis. This product is guaranteed for 1 year from date of receipt.

Citations for Recombinant Human HGF His Protein

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