Histone H1.2 Antibody (9T9Q7)

Novus Biologicals | Catalog # NBP3-15291

Recombinant Monoclonal Antibody
Novus Biologicals
100% Guarantee

Key Product Details

Species Reactivity

Human, Mouse, Rat

Applications

Western Blot, Immunocytochemistry/ Immunofluorescence

Label

Unconjugated

Antibody Source

Recombinant Monoclonal Rabbit IgG Clone # 9T9Q7 expressed in HEK293
View all Histone H1.2 Primary Antibodies »

Product Specifications

Immunogen

A synthetic peptide corresponding to a sequence within amino acids 1-100 of human Histone H1.2 (P16403). MSETAPAAPAAAPPAEKAPVKKKAAKKAGGTPRKASGPPVSELITKAVAASKERSGVSLAALKKALAAAGYDVEKNNSRIKLGLKSLVSKGTLVQTKGTG

Clonality

Monoclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for Histone H1.2 Antibody (9T9Q7)

Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291]

Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291]

Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Western blot analysis of extracts of various cell lines, using Histone H1.2 antibody (NBP3-15291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Basic Kit. Exposure time: 1s.
Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (9T9Q7) [NBP3-15291]

Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (9T9Q7) [NBP3-15291]

Immunocytochemistry/Immunofluorescence: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Immunofluorescence analysis of U-2 OS cells using Histone H1.2 Rabbit mAb (NBP3-15291) at dilution of 1:100 (40x lens). Blue: DAPI for nuclear staining.
Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291]

Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291]

Western Blot: Histone H1.2 Antibody (9T9Q7) [NBP3-15291] - Western blot analysis of extracts of various cell lines, using Histone H1.2 antibody (NBP3-15291) at 1:3000 dilution. Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) at 1:10000 dilution. Lysates/proteins: 25ug per lane. Blocking buffer: 3% nonfat dry milk in TBST. Detection: ECL Enhanced Kit. Exposure time: 180s.
Histone H1.2 Antibody (9T9Q7)

Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (9T9Q7) [Histone H1.2] -

Immunocytochemistry/ Immunofluorescence: Histone H1.2 Antibody (9T9Q7) [Histone H1.2] - Confocal imaging of U-2 OS cells using Histone H1.2 Rabbit mAb. The cells were counterstained with alpha-Tubulin Mouse mAb (Green). DAPI was used for nuclear staining (blue). Objective: 100x.

Applications for Histone H1.2 Antibody (9T9Q7)

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

1:50 - 1:500

Western Blot

1:1000 - 1:5000

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.3), 50% glycerol, 0.05% BSA

Preservative

0.02% Sodium Azide

Concentration

Please see the vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at -20C. Avoid freeze-thaw cycles.

Background: Histone H1.2

Histones are nuclear proteins that are responsible for the nucleosome structure of the chromosomal fiber as they play a critical role in transcription regulation, DNA repair, DNA replication, and chromosomal stability. The Histone H1.2 protein is 213 amino acids long, 21 kDA and is encoded by the HIST1H1C gene. H1 proteins are essential in forming chromatin fiber and for the condensation of nucleosome chains into higher-order structured fibers. The HIST1H1C gene has been investigated in leukemia and parkinson's disease. It plays a role in apoptosis, PKA signaling, cell cycle chromosome condensation in prometaphase, and histone modification through interactions with various genes such as TONSL, NPM1, YWHAQ, EED, and ESR2.

Alternate Names

H1 histone family, member 2, H1.2, H1F2H1C, histone 1, H1c, histone cluster 1, H1c, histone H1.2, Histone H1d, MGC3992

Gene Symbol

H1-2

Additional Histone H1.2 Products

Product Documents for Histone H1.2 Antibody (9T9Q7)

Certificate of Analysis

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Product Specific Notices for Histone H1.2 Antibody (9T9Q7)

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

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Protocols

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