HPF1 Antibody - BSA Free

Novus Biologicals | Catalog # NBP1-93973

Novus Biologicals
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Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Rat

Predicted:

Mouse (92%), Rat (91%). Backed by our 100% Guarantee.

Applications

Validated:

Western Blot, Immunocytochemistry/ Immunofluorescence, Simple Western

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Rabbit IgG

Format

BSA Free
View all HPF1 Primary Antibodies »

Product Specifications

Immunogen

This antibody was developed against Recombinant Protein corresponding to amino acids: LVGPYDILAGKHKTKKKSTGLNFNLHWRFYYDPPEFQTIIIGDNKTQYHMGYFRDSPDEFPVYVGINEAKKNCIIVPNGDNVFAA

Clonality

Polyclonal

Host

Rabbit

Isotype

IgG

Scientific Data Images for HPF1 Antibody - BSA Free

Western Blot: HPF1 Antibody [NBP1-93973]

Western Blot: HPF1 Antibody [NBP1-93973]

Western Blot: HPF1 Antibody [NBP1-93973] - Analysis in human cell line MOLT-4.
Immunocytochemistry/ Immunofluorescence: HPF1 Antibody [NBP1-93973]

Immunocytochemistry/ Immunofluorescence: HPF1 Antibody [NBP1-93973]

Immunocytochemistry/Immunofluorescence: HPF1 Antibody [NBP1-93973] - Staining of human cell line U-251 shows localization to nucleoli, nucleoplasm and cytosol. Antibody staining is shown in green.
Simple Western: HPF1 Antibody [NBP1-93973]

Simple Western: HPF1 Antibody [NBP1-93973]

Simple Western: HPF1 Antibody [NBP1-93973] - Simple Western lane view shows a specific band for C4orf27 in 0.2 mg/ml of MOLT-4 lysate(s). This experiment was performed under reducing conditions using the 12-230 kDa separation system.
Simple Western: HPF1 Antibody [NBP1-93973]

Simple Western: HPF1 Antibody [NBP1-93973]

Simple Western: HPF1 Antibody [NBP1-93973] - Electropherogram image of the corresponding Simple Western lane view. C4orf27 antibody was used at 1:25 dilution on MOLT-4 lysate(s) respectively.

Applications for HPF1 Antibody - BSA Free

Application
Recommended Usage

Immunocytochemistry/ Immunofluorescence

0.25-2 ug/ml

Simple Western

1:25

Western Blot

0.04-0.4 ug/ml
Application Notes
ICC/IF Fixation Permeabilization: Use PFA/Triton X-100.

In Simple Western only 10 - 15 uL of the recommended dilution is used per data point.
See Simple Western Antibody Database for Simple Western validation: Tested in MOLT-4 lysate(s), separated by Size, antibody dilution of 1:25, apparent MW was 47 kDa.

Formulation, Preparation, and Storage

Purification

Affinity purified

Formulation

PBS (pH 7.2) and 40% Glycerol

Format

BSA Free

Preservative

0.02% Sodium Azide

Concentration

Concentrations vary lot to lot. See vial label for concentration. If unlisted please contact technical services.

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store at 4C short term. Aliquot and store at -20C long term. Avoid freeze-thaw cycles.

Background: HPF1

Alternate Names

chromosome 4 open reading frame 27, FLJ20534, FLJ33423

Gene Symbol

C4orf27

Additional HPF1 Products

Product Documents for HPF1 Antibody - BSA Free

Certificate of Analysis

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Product Specific Notices for HPF1 Antibody - BSA Free

This product is for research use only and is not approved for use in humans or in clinical diagnosis. Primary Antibodies are guaranteed for 1 year from date of receipt.

Citations for HPF1 Antibody - BSA Free

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Protocols

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