Human ADAM12 Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB44161R Datasheet / COA / SDS

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FAB44161R-100UG

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Human ADAM12 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAM12 in direct ELISAs. In direct ELISAs, no cross-reactivity with recombinant human ADAM8, 9, 10, 15, 17, 19, 22, 23, 32, or 33 is observed.

Source

Monoclonal Mouse IgG1 Clone # 632525

Purification

Protein A or G purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAM12
Arg29-Ser513
Accession # O43184

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ADAM12

ADAM12 (A disintegrin and metalloprotease domain 12; also Meltrin-a) is a 120 kDa type I transmembrane glycoprotein member of the metzincin family of proteases. It is synthesized as a 909 amino acid (aa) preproprecursor that contains a 25 kDa proregion (aa 29-207) and a 92 kDa mature molecule. Proteolytic cleavage in the Golgi removes the propeptide prior to cell-surface expression. A 68 kDa soluble splice form exists in pregnancy where 36 amino acids replace the C-terminal 205 aa (705-909). This circulating ADAM12 isoform remains associated with its prodomain via noncovalent linkage, possibly acting as an enzyme regulator. Over aa 29-513, human ADAM12 shares 78% and 79% aa identity with bovine and mouse ADAM12, respectively.

Long Name

A Disintegrin and Metalloprotease-like Domain 12

Entrez Gene IDs

8038 (Human)

Alternate Names

a disintegrin and metalloproteinase domain 12 (meltrin alpha); ADAM 12; ADAM metallopeptidase domain 12; ADAM12; disintegrin and metalloproteinase domain-containing protein 12; EC 3.4.24; EC 3.4.24.58; MCMP; MCMPMltna; Meltrin alpha; meltrin-alpha; MLTNA; MLTNEC 3.4.24.-