Human ADAMTS13 Alexa Fluor® 647-conjugated Antibody

Catalog #: AF4245R Datasheet / COA / SDS

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AF4245R-100UG

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Human ADAMTS13 Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human ADAMTS13 in direct ELISAs and Western blots. In direct ELISAs, less than 1% cross‑reactivity with recombinant human (rh) ADAMTS14 is observed.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human ADAMTS13
Gln34-Trp688
Accession # Q76LX8

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

ELISA

Optimal dilution of this antibody should be experimentally determined.

Immunoprecipitation

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: ADAMTS13

ADAMTS13 (A disintegrin and metalloprotease with TSP motifs 13) is a 140-230 kDa, secreted glycoprotein member of the ADAMTS family of proteases. ADAMTS13 is produced by the liver and circulates in plasma where it cleaves and reduces the activity of von Willebrand factor. ProADAMTS13 is 1398 amino acids (aa) in length. It contains a 45 aa prodomain (aa 30-74), one peptidase domain (aa 80-286) and one disintegrin (aa 287-383) domain, multiple TSP regions and two CUB domains. The prodomain does not affect activity, while the TSP and CUB domains appear to be necessary for activity. One truncated form exists that shows truncation after Gln448. Over aa 34-688, human ADAMST13 shares 76% aa identity with mouse ADAMST13.

Long Name

A Disintegrin-like and Metalloproteinase Domain with Thrombospondin Motifs 13

Entrez Gene IDs

11093 (Human); 279028 (Mouse); 362091 (Rat)

Alternate Names

A disintegrin and metalloproteinase with thrombospondin motifs 13; a disintegrin-like and metalloprotease (reprolysin type) with thrombospondintype 1 motif, 13; ADAM metallopeptidase with thrombospondin type 1 motif, 13; ADAMTS13; ADAM-TS13; ADAMTS-13; DKFZp434C2322; EC 3.4.24.14; EC 3.4.24.82; EC 3.4.24.87; FLJ42993; MGC118899; MGC118900; TTP; TTPADAM-TS 13; vWF-cleaving protease; vWF-CP; vWF-CPC9orf8; VWFCPvon Willebrand factor-cleaving protease