Human Adiponectin/Acrp30 Antibody

R&D Systems | Catalog # AF1065

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, Simple Western

Cited:

Immunohistochemistry, Immunohistochemistry-Paraffin, Western Blot, Neutralization, ELISA Capture, Native SDS-PAGE

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Adiponectin/Acrp30 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Adiponectin/Acrp30
Glu19-Asn244
Accession # Q15848

Specificity

Detects human Adiponectin/Acrp30 in direct ELISAs and Western blots. In direct ELISAs, less than 30% cross‑reactivity with recombinant mouse Adiponectin is observed and less than 15% cross-reactivity with recombinant rat Adiponectin is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Adiponectin/Acrp30 Antibody

Detection of Human Adiponectin/Acrp30 antibody by Western Blot.

Detection of Human Adiponectin/Acrp30 by Western Blot.

Western blot shows lysates of human placenta tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Adiponectin/Acrp30 at approximately 30-32 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Human Adiponectin/Acrp30 antibody by Simple WesternTM.

Detection of Human Adiponectin/Acrp30 by Simple WesternTM.

Simple Western lane view shows lysates of human placenta tissue, loaded at 0.2 mg/mL. A specific band was detected for Adiponectin/Acrp30 at approximately 41 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Adiponectin/Acrp30 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF1065) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Adiponectin/Acrp30 by Western Blot

Detection of Adiponectin/Acrp30 by Western Blot

ISM1 regulates adiponectin expression in the lungs. (A) Relative adiponectin protein expression in lung lysates (n = 5). Data are presented as the mean ± SD. *P < 0.05, using Student’s t test. (B) Adiponectin level in the BALF of HDM-challenged mice compared with the saline control measured with ELISA (n = 5). Data are presented as the mean ± SD. ****P < 0.0001, using two-way ANOVA. (C) Immunofluorescence images showing colocalization between adiponectin and SP-C in WT lungs. Bars = 20 μm. (D) Adiponectin expression in conditioned media from A549 cells treated with different doses of rISM1. (E-F) PPAR gamma protein expression and quantification in A549 cell lysates post-treated with 200 nM of rISM1 measured at different time points. (G-H) Adiponectin expression and quantification in A549 conditioned media under 200 nM rISM1 with or without GRP78 antibody neutralization. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, using one-way ANOVA. SP-C, surfactant protein C; PPAR gamma, peroxisome proliferator-activated receptor gamma; GRP78, glucose-regulating protein 78 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37932719), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Adiponectin/Acrp30 by Western Blot

Detection of Adiponectin/Acrp30 by Western Blot

ISM1 regulates adiponectin expression in the lungs. (A) Relative adiponectin protein expression in lung lysates (n = 5). Data are presented as the mean ± SD. *P < 0.05, using Student’s t test. (B) Adiponectin level in the BALF of HDM-challenged mice compared with the saline control measured with ELISA (n = 5). Data are presented as the mean ± SD. ****P < 0.0001, using two-way ANOVA. (C) Immunofluorescence images showing colocalization between adiponectin and SP-C in WT lungs. Bars = 20 μm. (D) Adiponectin expression in conditioned media from A549 cells treated with different doses of rISM1. (E-F) PPAR gamma protein expression and quantification in A549 cell lysates post-treated with 200 nM of rISM1 measured at different time points. (G-H) Adiponectin expression and quantification in A549 conditioned media under 200 nM rISM1 with or without GRP78 antibody neutralization. Data are presented as the mean ± SD. *P < 0.05; **P < 0.01; ***P < 0.001; ****P < 0.0001, using one-way ANOVA. SP-C, surfactant protein C; PPAR gamma, peroxisome proliferator-activated receptor gamma; GRP78, glucose-regulating protein 78 Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/37932719), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Adiponectin/Acrp30 Antibody

Application
Recommended Usage

Simple Western

10 µg/mL
Sample: Human placenta tissue

Western Blot

1 µg/mL
Sample: Human placenta tissue

Reviewed Applications

Read 1 review rated 4 using AF1065 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Adiponectin/Acrp30

Adiponectin, also known as Acrp30, is an adipocyte-derived protein with wide ranging paracrine and endocrine effects on metabolism and inflammation. It is induced during adipocyte differentiation, and its secretion is stimulated by insulin. It promotes adipocyte differentiation, fatty acid catabolism, and insulin sensitivity and is negatively correlated with obesity, type 2 diabetes, and atherogenesis. In this context, Adiponectin is an anti-inflammatory agent, but it exerts pro-inflammatory effects in nonmetabolic disorders such as rheumatoid arthritis and inflammatory bowel disease (1-3). Adiponectin interacts with the receptors AdipoR1 and AdipoR2, calreticulin, and Cadherin-13/T-Cadherin, as well as with several growth factors (4-7). Mature human Adiponectin consists of a 60 amino acid (aa) N-terminal collagenous region and a 137 aa C-terminal C1q-like globular domain which can be cleaved by a leukocyte-derived elastase (8-9). Mature human Adiponectin shares 83% and 85% amino acid (aa) sequence identity with mouse and rat Adiponectin, respectively. Adiponectin associates into trimers that may assemble into medium molecular weight (MMW) hexamers and then into > 300 kDa high molecular weight (HMW) oligomers (10-12). The glycosylation of four hydroxylated lysine residues in the collagenous domain is required for the intracellular formation of HMW complexes (13). The various multimeric forms of Adiponectin exhibit distinct tissue specific and gender specific profiles and activities (12, 14).

References

  1. Lara-Castro, C. et al. (2007) Curr. Opin. Lipidol. 18:263.
  2. Tilg, H. and A.R. Moschen (2006) Nat. Rev. Immunol. 6:772.
  3. Fantuzzi, G. (2008) J. Allergy Clin. Immunol. 121:326.
  4. Yamauchi, T. et al. (2007) Nat. Med. 13:332.
  5. Takemura, Y. et al. (2007) J. Clin. Invest. 117:375.
  6. Hug, C. et al. (2004) Proc. Natl. Acad. Sci. 101:10308.
  7. Wang, Y. et al. (2005) J. Biol. Chem. 280:18341.
  8. Maeda, K. et al. (1996) Biochem. Biophys. Res. Commun. 221:286.
  9. Waki, H. et al. (2005) Endocrinology 146:790.
  10. Waki, H. et al. (2003) J. Biol. Chem. 278:40352.
  11. Tsao, T.S. et al. (2003) J. Biol. Chem. 278:50810.
  12. Wang, Y. et al. (2008) Biochem. J. 409:623.
  13. Wang, H. et al. (2006) J. Biol. Chem. 281:16391.
  14. Pajvani, U.B. et al. (2003) J. Biol. Chem. 278:9073.

Alternate Names

Acrp30, AdipoQ, ApM1, GBP28

Entrez Gene IDs

9370 (Human); 11450 (Mouse); 246253 (Rat)

Gene Symbol

ADIPOQ

UniProt

Q15848

Additional Adiponectin/Acrp30 Products

Product Documents for Human Adiponectin/Acrp30 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Adiponectin/Acrp30 Antibody

For research use only

Citations for Human Adiponectin/Acrp30 Antibody

Customer Reviews for Human Adiponectin/Acrp30 Antibody (1)

4 out of 5
1 Customer Rating
5 Stars
0%
4 Stars
100%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Adiponectin/Acrp30 Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 for a review with an image

$10/€7/£6/$10CAN/¥1110 for a review without an image

Submit a review
Amazon Gift Card
Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Name: Anonymous
    Application: Immunohistochemistry-Paraffin
    Sample Tested: See PMID 21935928
    Species: Human
    Verified Customer | Posted 01/05/2015

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies