Human Akt3 Antibody (199864)

Name: Human Akt3 Antibody (199864)
Brand: R&D Systems
SKU: MAB1463
Price: 439 USD
Availability: InStock

R&D Systems | Catalog # MAB1463

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Product Specifications
Scientific Data
Applications
Preparation & Storage
Calculators
Background
Product Documents
Specific Notices
Research Areas

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG₁ Clone # 199864

View all Akt3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Akt3
Met1-Glu479
Accession # Q9Y243

Specificity

Detects endogenous human Akt3 in Western blots. Using Western blots, this antibody does not detect recombinant human Akt1 or Akt2.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG₁

Scientific Data Images for Human Akt3 Antibody (199864)

Detection of Human Akt3 by Western Blot.

Western blot shows lysates of DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line. PVDF membrane was probed with 0.5 µg/mL of Human Akt3 Monoclonal Antibody (Catalog # MAB1463) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Akt3 at approximately 60 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 3.

Applications for Human Akt3 Antibody (199864)

Application

Recommended Usage

Western Blot

0.5 µg/mL
Sample: DU145 human prostate carcinoma cell line and MDA-MB-231 human breast cancer cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

Volume (to add to vial)

Mass (in vial)

Desired Reconstitution Concentration

Background: Akt3

Akt3 (v-akt murine thymoma viral oncogene homolog 3), also known as protein kinase B gamma (PKB gamma ) and RAC-gamma serine/threonine kinase (RAC-PK-gamma ), is a member of the Akt family that also includes Akt1 (PKB alpha ) and Akt2 (PKB beta ). These highly homologous kinases are central in such diverse cellular processes as glucose uptake, cell cycle progression, and apoptosis. All three Akts contain an amino-terminal pleckstrin homology domain, a central kinase domain, and a carboxyl-terminal regulatory domain.