Human alpha 2-Macroglobulin Antibody Summary
*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Applications
Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.
Scientific Data
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Detection of Human alpha 2‑Macroglobulin by Western Blot. Western blot shows human serum. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human a2-Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for a2-Macroglobulin at approximately 180 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
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Detection of Human alpha 2‑Macroglobulin by Simple WesternTM. Simple Western lane view shows lysates of Exosome Standards (HT‑29) (NBP3-11685) and human serum, loaded at 0.5 mg/ml and 1:10 respectively. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/ml of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938) followed by HRP-conjugated Goat Anti-Mouse Secondary Antibody (Catalog # 042-205). This experiment was conducted under reducing conditions and using the 12-230kDa separation system.
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Detection of Human alpha 2‑Macroglobulin by Simple WesternTM. Simple Western lane view shows human serum, loaded at 0.2 mg/mL. A specific band was detected for alpha 2‑Macroglobulin at approximately 178 kDa (as indicated) using 10 µg/mL of Mouse Anti-Human alpha 2‑Macroglobulin Monoclonal Antibody (Catalog # MAB1938). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Reconstitution Calculator
Preparation and Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Background: alpha 2-Macroglobulin
Alpha 2-macroglobulin (A2M) is a general and irreversible protease inhibitor implicated in many processes. It is able to inhibit all four classes of proteases by a unique trapping mechanism.
Product Datasheets
Citations for Human alpha 2-Macroglobulin Antibody
R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.
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Citations: Showing 1 - 3
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Fecal host biomarkers predicting severity of Clostridioides difficile infection
Authors: M Golizeh, K Winter, L Roussel, M Landekic, M Langelier, VG Loo, M Ndao, DC Vinh
JCI Insight, 2021-01-11;0(0):.
Species: Human
Sample Types: Protein
Applications: Western Blot -
Maggot chymotrypsin I from Lucilia sericata is resistant to endogenous wound protease inhibitors.
Authors: Telford G, Brown AP, Kind A, English JS, Pritchard DI
Br. J. Dermatol., 2010-11-29;164(1):192-6.
Species: Human
Sample Types: Tissue Homogenates
Applications: Western Blot -
Anti-Ebola MAb 17A3 reacts with bovine and human alpha-2-macroglobulin proteins
Authors: Jae-Sung Yu, Ben-Jiang Ma, Richard M. Scearce, Hua-Xin Liao, Barton F. Haynes
Journal of Virological Methods
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