Human AMPK beta 2 Antibody

Catalog #: MAB11639 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB11639-100
MAB11639-SP

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Detection of Human AMPK beta 2 by Western Blot.

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All AMPK beta 2 Antibodies

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human AMPK beta 2 Antibody Summary

Species Reactivity

Human

Specificity

Detects recombinant human AMPK beta 2 protein in Direct ELISA.

Source

Monoclonal Mouse IgG_2B Clone # 1092615

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

E. coli- derived recombinant human AMPKbeta2
Met1-Ile272
Accession # O43741

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

HepG2 human hepatocellular carcinoma cell line, NTera-2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma

Simple Western

20 µg/mL

HEK293T human embryonic kidney cell line

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human AMPK beta 2 by Western Blot. Western Blot shows lysates of HepG2 human hepatocellular carcinoma cell line, NTera‑2 human testicular embryonic carcinoma cell line and U2OS human osteosarcoma cell line. PVDF membrane was probed with 2 µg/ml of Mouse Anti-Human AMPK beta 2 Monoclonal Antibody (Catalog # MAB11639) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for AMPK beta 2 at approximately 32 kDa (as indicated). This experiment was conducted under reducing conditions and using Western Blot Buffer Group 1.

Simple Western

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Detection of Human AMPK beta 2 by Simple Western^TM. Simple Western shows lysates of HEK293T human embryonic kidney cell line, loaded at 0.5 mg/ml. A specific band was detected for AMPK beta 2 at approximately 38 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human AMPK beta 2 Monoclonal Antibody (Catalog # MAB11639). This experiment was conducted under reducing conditions and using the 12‑230 kDa separation system.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute lyophilized material at 0.2 mg/ml in sterile PBS. For liquid material, refer to CoA for concentration.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: AMPK beta 2

AMP-activated protein kinase (AMPK) is a heterotrimeric complex consisting of a catalytic alpha subunit and regulatory beta and gamma subunits. Each subunit exists as alternate isoforms ( alpha 1, alpha 2, beta 1, beta 2, gamma 1, gamma 2, gamma 3), with all 12 combinations able to form complexes. The beta 1 subunit is expressed at higher levels than the beta 2subunit in liver, while beta 2 is more abundant in skeletal muscle. AMPK’s role in metabolic regulation has implicated this serine/threonine kinase as a therapeutic target in heart disease, obesity, and diabetes.

Long Name

AMP-activated Protein Kinase beta 2

Entrez Gene IDs

5565 (Human); 108097 (Mouse); 64562 (Rat)

Alternate Names

5'-AMP-activated protein kinase, beta-2 subunit; AMPK beta 2; AMPK beta 2,5'-AMP-activated protein kinase subunit beta-2; AMPK beta-2 chain; AMPK subunit beta-2; MGC61468; PRKAB2; protein kinase, AMP-activated, beta 2 non-catalytic subunit