Human Arylsulfatase B/ARSB Antibody (708401)

R&D Systems | Catalog # MAB4415

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 708401
View all Arylsulfatase B/ARSB Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human Arylsulfatase B/ARSB
Ser37-Met533
Accession # P15848

Specificity

Detects human Arylsulfatase B/ARSB in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human (rh) ARSA, rhARSJ, or recombinant mouse ARSG is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Arylsulfatase B/ARSB Antibody (708401)

Detection of Human Arylsulfatase B/ARSB antibody by Western Blot.

Detection of Human Arylsulfatase B/ARSB by Western Blot.

Western blot shows lysates of HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Arylsulfatase B/ARSB at approximately 47 to 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Arylsulfatase B/ARSB antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Arylsulfatase B/ARSB in HepG2 Human Cell Line.

Arylsulfatase B/ARSB was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Arylsulfatase B/ARSB Monoclonal Antibody (Catalog # MAB4415) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Arylsulfatase B/ARSB Antibody (708401)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HepG2 human hepatocellular carcinoma cell line, Huh‑7 human hepatoma cell line, and human skeletal muscle tissue

Reviewed Applications

Read 1 review rated 5 using MAB4415 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Arylsulfatase B/ARSB

Arylsulfatase B (ARSB) is a lysosomal enzyme of the sulfatase family that functions as an N-acetylgalactosamine-4-sulfatase (1). The enzyme is also active against chondroitin sulfate and dermatan sulfate (2). Deficiencies in ARSB activity are responsible for the lysosomal storage disease mucopolysaccharidosis Type VI, also known as Maroteaux-Lamy Syndrome (3).

References

  1. Peters, C. et al. (1990) J. Biol. Chem. 265:3374.
  2. O’Brien, J.S. et al. (1974) Biochem. Biophys. Res. Commun. 60:1170.
  3. Wicker, G. et al. (1991) J. Biol. Chem. 266:21386.

Alternate Names

ARSB, ASB, N-acetylgalactosamine-4-sulfatase

Entrez Gene IDs

411 (Human); 11881 (Mouse); 25227 (Rat)

Gene Symbol

ARSB

UniProt

P15848

Additional Arylsulfatase B/ARSB Products

Product Documents for Human Arylsulfatase B/ARSB Antibody (708401)

Certificate of Analysis

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Product Specific Notices for Human Arylsulfatase B/ARSB Antibody (708401)

For research use only

Citations for Human Arylsulfatase B/ARSB Antibody (708401)

Customer Reviews for Human Arylsulfatase B/ARSB Antibody (708401) (1)

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  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22902879
    Species: Human
    Verified Customer | Posted 02/20/2015

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