Human Bag-1 Antibody (GP3.10G3E2)

R&D Systems | Catalog # MAB852

R&D Systems

Key Product Details

Validated by

Knockout/Knockdown

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Knockout Validated, Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # GP3.10G3E2
View all Bag-1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Bag-1

Specificity

Detects the 36 kDa and 50 kDa isoforms of human Bag-1. Does not detect mouse Bag-1.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Bag-1 Antibody (GP3.10G3E2)

Detection of Human Bag‑1 antibody by Western Blot.

Detection of Human Bag‑1 by Western Blot.

Western blot shows lysates of THP-1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line. PVDF Membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). Specific bands were detected for Bag-1 at approximately 36 and 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 6.
Immunoprecipitation of Human Bag‑1.

Immunoprecipitation of Human Bag‑1.

Bag-1 was immunoprecipitated from lysates (5 x 106cells) of THP-1 human acute monocytic leukemia and HEK293 human embryonic kidney cell line following incubation with 3 µg Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) for 1-16 hours at 4 °C. Bag-1-antibody complexes were absorbed using Protein A Immunoprecipitin (Life Technologies). Immuno-precipitated Bag-1 (left panel) was detected by Western blot using 0.5 µg/mL Mouse CD30/TNFRSF8 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF852). View our recommended buffer recipes for immunoprecipitation.
Western Blot Shows Human Bag-1 Antibody Specificity by Using Knockout Cell Line.

Western Blot Shows Human Bag‑1 Specificity by Using Knockout Cell Line.

Western blot shows lysates of HeLa human cervical epithelial carcinoma parental cell line and Bag-1 knockout HeLa cell line (KO). PVDF membrane was probed with 0.5 µg/mL of Mouse Anti-Human Bag-1 Monoclonal Antibody (Catalog # MAB852) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). Specific bands were detected for Bag-1 at approximately 29, 39, and 50 kDa (as indicated) in the parental HeLa cell line, but is not detectable in knockout HeLa cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Bag-1 Antibody (GP3.10G3E2)

Application
Recommended Usage

Immunoprecipitation

0.6 µg/106 cells
Sample: THP‑1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line, see our available Western blot detection antibodies

Knockout Validated

Bag‑1 is specifically detected in HeLa human cervical epithelial carcinoma parental cell line but is not detectable in Bag‑1 knockout HeLa cell line.

Western Blot

0.5 µg/mL
Sample: THP‑1 human acute monocytic leukemia cell line and HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Bag-1

Bag-1 (Bcl-2-associated Athanogene 1) is a 31-kDa protein with an apparent molecular mass of 46 kDa that can bind to Bcl-2 and enhance its anti-apoptotic effect, resulting in increased protection from cell death induced by apoptotic stimuli. Bag-1 shares no significant homology with Bcl-2 or other Bcl-2 family proteins. The C-terminal region of Bag-1 binds the PDGF and HGF Receptor, and enhances growth factor-mediated protection from apoptosis. This represents a link between growth factor receptors and anti-apoptotic mechanisms.

Long Name

Bcl-2-associated Anthanogene-1

Alternate Names

Bag1

Entrez Gene IDs

573 (Human); 12017 (Mouse)

Gene Symbol

BAG1

Additional Bag-1 Products

Product Documents for Human Bag-1 Antibody (GP3.10G3E2)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Bag-1 Antibody (GP3.10G3E2)

For research use only

Related Research Areas

Bcl-2 Proteins and Regulators

Citations for Human Bag-1 Antibody (GP3.10G3E2)

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