Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Sheep IgG
View all CapG Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CapG
Met1-Lys348
Accession # P40121

Specificity

Detects human CapG in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Sheep

Isotype

IgG

Scientific Data Images for Human CapG Antibody

Detection of Human CapG antibody by Western Blot.

Detection of Human CapG by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line, MOLT-4 human acute lymphoblastic leukemia cell line, and human kidney tissue. PVDF membrane was probed with 0.2 µg/mL of Sheep Anti-Human CapG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7177) followed by HRP-conjugated Anti-Sheep IgG Secondary Antibody (Catalog # HAF016). A specific band was detected for CapG at approximately 41 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
CapG antibody in U937 Human Cell Line by Immunocytochemistry (ICC).

CapG in U937 Human Cell Line.

CapG was detected in immersion fixed U937 human histiocytic lymphoma cell line using Sheep Anti-Human CapG Antigen Affinity-purified Polyclonal Antibody (Catalog # AF7177) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Sheep IgG Secondary Antibody (red; Catalog # NL010) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Applications for Human CapG Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed U937 human histiocytic lymphoma cell line

Western Blot

0.2 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line, MOLT‑4 human acute lymphoblastic leukemia cell line, and human kidney tissue

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Sterile PBS to a final concentration of 0.2 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CapG

CapG ("Caps" barbed actin ends and resembles Gelsolin; also Macrophage capping protein/MCP and actin regulatory protein Cap-G) is a 40-42 kDa member of the Villin/Gelsolin family of proteins. It is expressed by macrophages/monocytes and endothelial cells, and is found in both cytoplasm and nucleus. It appears to be involved in cell motility, and is known to bind to (but not cleave) actin filament ends in a calcium-dependent manner. This regulates the growth of actin filaments. Human CapG is 348 amino acids (aa) in length. It contains an N-terminal gelsolin repeat (aa 27-75), followed by a NLS (aa 137-146), two consecutive gelsolin-like repeats (aa 148-188 and 261-307), and one utilized phosphorylation site at Ser337. There is at least one potential isoform variant that contains a deletion of aa 64-84. Full-length human CapG shares 91% aa sequence identity with mouse CapG.

Long Name

Capping Protein, Gelsolin-like

Alternate Names

AFCP

Entrez Gene IDs

822 (Human); 12332 (Mouse); 297339 (Rat)

Gene Symbol

CAPG

UniProt

P40121 (Human)

Additional CapG Products

Product Documents for Human CapG Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CapG Antibody

For research use only

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Protocols

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