Human Carboxypeptidase A1/CPA1 Antibody (477305)

R&D Systems | Catalog # MAB2856

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG2B Clone # 477305
View all Carboxypeptidase A1/CPA1 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Carboxypeptidase A1/CPA1
Lys17-Tyr419
Accession # P15085

Specificity

Detects human Carboxypeptidase A1/CPA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, 25% cross-reactivity with recombinant mouse (rm) Carboxypeptidase A1 is observed and no cross-reactivity with recombinant human (rh) Carboxypeptidase A2, B1, E, or recombinant mouse Carboxypeptidase A4 is observed.

Clonality

Monoclonal

Host

Rat

Isotype

IgG2B

Scientific Data Images for Human Carboxypeptidase A1/CPA1 Antibody (477305)

Detection of Carboxypeptidase A1 by Western Blot

Detection of Carboxypeptidase A1 by Western Blot

Effect of the p.G250A CPA1 variant on cellular secretion, enzyme activity, intracellular protein levels, solubility, and BiP protein levels in transfected HEK 293T cells. For comparison, wild-type CPA1 (WT), the reference pathogenic mutation p.N256K, and empty expression vector were studied. (A) Secretion of proCPA1 to the growth medium analyzed by SDS-PAGE and Coomassie Blue staining. CPA1 enzyme activity in the medium after activation of proCPA1 is indicated in milliOD/min units. (B) Protein levels of proCPA1, BiP, and alpha -tubulin in total cell lysates, and in the insoluble and soluble cellular fractions. Proteins were detected by Western blotting. (C) Densitometric evaluation of proCPA1 and BiP levels in total cell lysates, and in the insoluble and soluble cellular fractions. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/36555104), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Carboxypeptidase A1/CPA1 Antibody (477305)

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Carboxypeptidase A1/CPA1 (Catalog # 2856-ZN)

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Carboxypeptidase A1/CPA1

Carboxypeptidase A1 encoded by the CPA1 gene cleaves the C-terminal amide or ester bond of peptides that have a free C-terminal carboxyl group (1). It prefers the C-terminal residues with aromatic or branched aliphatic side chains including Phe, Tyr, Trp, Leu or Ile. It is important in the degradation of food proteins to produce essential amino acids such as Phe and Trp. The deduced amino acid sequence of human CPA1 consists of a signal peptide (residues 1 to 16), a pro region (residue 17 to 110), and a mature chain (residues 111 to 419). The purified rhCPA1 corresponds to the pro form.

References

  1. Auld, D.S. (2004) in Handbook of Proteolytic Enzymes (ed. Barrett, et al.) pp. 812, Academic Press, San Diego.

Alternate Names

CPA1

Entrez Gene IDs

1357 (Human); 109697 (Mouse)

Gene Symbol

CPA1

UniProt

P15085

Additional Carboxypeptidase A1/CPA1 Products

Product Documents for Human Carboxypeptidase A1/CPA1 Antibody (477305)

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Carboxypeptidase A1/CPA1 Antibody (477305)

For research use only

Related Research Areas

Metalloproteases and Regulators

Citations for Human Carboxypeptidase A1/CPA1 Antibody (477305)

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