Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 212211
View all Cathepsin E Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Cathepsin E
Gln18-Pro396
Accession # NP_001901

Specificity

Detects human Cathepsin E in direct ELISAs and Western blots. It recognizes both the pro and mature forms of recombinant human (rh) Cathepsin E. In Western blots, 100% cross‑reactivity with recombinant mouse (rm) Cathepsin E and recombinant human (rh) BACE is observed under reducing conditions. Under non-reducing conditions, less than 1% cross-reactivity with rmCathepsin E and rhBACE-1 is observed. Less than 1% cross-reactivity with rhCathepsin D is observed under both reducing and non-reducing conditions.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Cathepsin E Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Cathepsin E (Catalog # 1294-AS), see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Cathepsin E (Catalog # 1294-AS)

Reviewed Applications

Read 1 review rated 3 using MAB1294 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cathepsin E

Cathepsin E is an intracellular aspartic protease of the pepsin family (1). Unlike Cathepsin D, another member of the same family and a lysosomal protease with relatively ubiquitous distribution, Cathepsin E is not a lysosomal enzyme and has a limited cell and tissue distribution. However, both Cathepsin D and E play an important role in the degradation of proteins, the generation of bioactive proteins, and antigen processing (2). Both enzymes are efficient in cleaving Swedish mutant of amyloid precursor protein (APP) at the beta  site but show almost no reactivity with wild-type APP (3). Human Cathepsin E is synthesized as a precursor protein, consisting of a signal peptide (aa 1‑17), a propeptide (aa 18‑53), and a mature chain (aa 54‑396) (4).

References

  1. Kay, J. and P.J. Tatnell (2004) in Handbook of Proteolytic Enzymes (Barrett, A.J. et al. eds.), p. 33, Academic Press, San Diego.
  2. Tsukuba, T. et al. (2000) Mol. Cells 10:601.
  3. Gruninger-Leitch, F. et al. (2000) Nat. Biotechnol. 18:66.
  4. Azuma, T. et al. (1989) J. Biol. Chem. 264:16748.

Alternate Names

CTSE

Entrez Gene IDs

1510 (Human); 13034 (Mouse)

Gene Symbol

CTSE

UniProt

NP_001901

Additional Cathepsin E Products

Product Documents for Human Cathepsin E Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cathepsin E Antibody

For research use only

Customer Reviews for Human Cathepsin E Antibody (1)

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  • Human Cathepsin E Antibody
    Name: Sam Ivry
    Application: Western Blot
    Sample Tested: Recombinant protein and Adult pancreas
    Species: Human
    Verified Customer | Posted 04/21/2016
    1:500 dilution in 5% milk (PBS with Tween) Lane 1: Ladder Lane 2: Recombinant CTSE Lane 3: Activated recombinant CTSE Lane 4: Adult pancreas
    Human Cathepsin E Antibody MAB1294

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