Rat anti-Mouse IgG2A Secondary Antibody (344701)

R&D Systems | Catalog # MAB0032

R&D Systems

Key Product Details

Species Reactivity

Mouse

Applications

Flow Cytometry

Label

Unconjugated

Antibody Source

Monoclonal Rat IgG1 Clone # 344701
View all IgG2A Secondary Antibodies »

Product Specifications

Immunogen

Cocktail of mouse IgG1 and IgG2A

Specificity

The specificity of this antibody was confirmed by direct ELISA on mouse IgG

Clonality

Monoclonal

Host

Rat

Isotype

IgG1

Scientific Data Images for Rat anti-Mouse IgG2A Secondary Antibody (344701)

Detection of Mouse IgG2A Primary Antibody by Flow Cytometry

Detection of Mouse IgG2A Primary Antibody by Flow Cytometry

Human peripheral blood lymphocytes were stained with A) Mouse Anti-Human CD4 Monoclonal Antibody (Catalog # MAB3791, Mouse IgG2A, filled histogram) or isotype control (Catalog # MAB003, open histogram), or B) Mouse Anti-Human CD8 alpha Monoclonal Antibody(Catalog # MAB1509, Mouse IgG2B, filled histogram) or isotype control (Catalog # MAB0041, open histogram), followed by Rat Anti-Mouse IgG2A isotype-specific secondary antibody (Catalog # MAB0032) and then Allophycocyanin-conjugated Anti-Rat IgG SecondaryAntibody (Catalog # F0113). View our protocol for Staining Membrane-associated Proteins.

Applications for Rat anti-Mouse IgG2A Secondary Antibody (344701)

Application
Recommended Usage

Flow Cytometry

0.25 µg/106 cells
Sample: Mouse IgG2A Primary Antibody

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Flow Cytometry

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose.

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: IgG2A

IgG is the most abundant of the five classes of mammalian immunoglobulins in serum. IgG molecules (~150 kDa) consist of two ~50 kDa heavy chains and two 25 kDa light chains connected by disulfide bonds. Antibody specificity is conferred by a combination of somatic rearrangement and hypermutation within variable regions. Mouse IgG isotypes IgG1, IgG2a and IgG2b share approximately 64-78% amino acid sequence identity within the heavy chain constant region domains (CH1, CH2 and CH3) and may vary in their interaction with complement, NK and mast cells, induction or inhibition by specific cytokines, and ability to cross the placenta.

Long Name

Immunoglobulin G2A Fc

Additional IgG2A Products

Product Documents for Rat anti-Mouse IgG2A Secondary Antibody (344701)

Certificate of Analysis

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Product Specific Notices for Rat anti-Mouse IgG2A Secondary Antibody (344701)

For research use only

Citations for Rat anti-Mouse IgG2A Secondary Antibody (344701)

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