Human CCL1/I-309/TCA-3 Antibody (35305)

R&D Systems | Catalog # MAB272

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Neutralization, Immunocytochemistry, ELISA Capture, ELISA Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 35305
View all CCL1/I-309/TCA-3 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL1/I‑309/TCA‑3
Lys24-Lys96
Accession # P22362

Specificity

Detects human CCL1/I‑309/TCA‑3 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human CCL2, 3, 4, 5, 7, 8, 9/10/MIP-1 gamma, 11, 14, 17, 19, 20, 21, 25, 28, recombinant mouse CCL2, 3, 5, 6, 7, 9/10/MIP-1 gamma, 11, 21, or 25 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL1/I-309/TCA-3 Antibody (35305)

Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Anti-body.

Chemotaxis Induced by CCL1/I‑309 and Neutralization by Human CCL1/I‑309 Anti-body.

Recombinant Human CCL1/I-309 (Catalog # 272-I) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR8 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL1/I-309 (0.02 µg/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL1/I-309 Monoclonal Antibody (Catalog # MAB272). The ND50 is typically 0.1-0.5 µg/mL.
Human CCL1 / I-309 / TCA-3 ELISA Standard Curve

Human CCL1 / I-309 / TCA-3 ELISA Standard Curve

Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I) was serially diluted and captured by Mouse Anti-Human CCL1/I‑309/TCA‑3 Monoclonal Antibody (Catalog # MAB272) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL1/I‑309/TCA‑3 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF272) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL1/I-309/TCA-3 Antibody (35305)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL1/I‑309/TCA‑3 (Catalog # 272-I)

Neutralization

Measured by its ability to neutralize CCL1/I‑309/TCA‑3-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR8. The Neutralization Dose (ND50) is typically 0.1-0.5 µg/mL in the presence of 0.02 µg/mL Recombinant Human CCL1/I‑309/TCA‑3.

Human CCL1/I-309 Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL1/I‑309/TCA‑3 Biotinylated Antibody (Catalog # BAF272)
  • Standard: Recombinant Human CCL1/I-309 Protein (Catalog # 272-I)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Reviewed Applications

Read 1 review rated 4 using MAB272 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from ascites

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL1/I-309/TCA-3

Human CCL1 was initially identified by subtractive hybridization as a transcript that was present in a gamma / delta T cell line but not in EBV-transformed B cells. Human CCL1 has been assumed to be a homologue of the mouse TCA3. While the two proteins share only approximately 42% amino acid sequence identity, both chemokines contain an extra pair of cysteine residues not found in most other chemokines. Human CCL1 and mouse TCA3 also share significant sequence homology in the 5’ flanking region of their genes. CCL1 cDNA encodes a 96 amino acid precursor protein with a hydrophobic signal peptide that is cleaved to yield a 73 amino acid mature protein. The CCL1 sequence contains one potential N‑linked glycosylation site, and natural CCL1 secreted by activated T cells is a glycoprotein doublet of 15‑16 kDa. The amino acid sequence of CCL1 identified the protein as a member of the chemokine beta subfamily.

References

  1. Miller, M.D. and M.S. Krangel (1992) Proc. Natl. Acad. Sci. USA 89:2950.
  2. Miller, M.D. et al. (1990) J. Immunol. 145:2737.

Alternate Names

I-309, P500, SCYA1, SCYA2, TCA-3, TCA3

Entrez Gene IDs

6346 (Human); 20290 (Mouse)

Gene Symbol

CCL1

UniProt

P22362

Additional CCL1/I-309/TCA-3 Products

Product Documents for Human CCL1/I-309/TCA-3 Antibody (35305)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL1/I-309/TCA-3 Antibody (35305)

For research use only

Citations for Human CCL1/I-309/TCA-3 Antibody (35305)

Customer Reviews for Human CCL1/I-309/TCA-3 Antibody (35305) (1)

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  • Human CCL1/I-309/TCA-3 Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Serum and Plasma
    Species: Human
    Verified Customer | Posted 11/22/2017
    MAB272 was used as the capture and BAF272 as the detection in a sandwich immunoassay. The immunoassay standard was 272-I. It worked very well. Most healthy human samples were not detectable.
    Human CCL1/I-309/TCA-3 Antibody (35305) MAB272

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