Human CCL13/MCP-4 Antibody

Catalog #: AF327
3 Citations Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF327
AF327-SP

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Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/MCP‑4 Antibody.

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All CCL13/MCP-4 Antibodies

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Citations (3)

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human CCL13/MCP-4 Antibody Summary

Species Reactivity

Human

Specificity

Detects human CCL13/MCP-4 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CCL13/MCP-4
Gln24-Thr98
Accession # Q99616

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

0.1 µg/mL

Recombinant Human CCL13/MCP‑4 (Catalog # 327-P4)

Neutralization

Measured by its ability to neutralize CCL13/MCP‑4-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR2A. The Neutralization Dose (ND₅₀) is typically 15-40 µg/mL in the presence of 2 µg/mL Recombinant Human CCL13/MCP‑4.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Neutralization

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Chemotaxis Induced by CCL13/MCP‑4 and Neutralization by Human CCL13/MCP‑4 Antibody. Recombinant Human CCL13/MCP-4 (Catalog # 327-P4) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR2A in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (AR002). Chemotaxis elicited by Recombinant Human CCL13/MCP-4 (2 µg/mL) is neutralized (green line) by increasing concentrations of Goat Anti-Human CCL13/MCP-4 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF327). The ND₅₀ is typically 15-40 µg/mL.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: CCL13/MCP-4

Human CCL13 is a CC chemokine recently cloned from a human fetal and a human heart cDNA library. Human CCL13 cDNA encodes a 98 amino acid residue precursor protein with a 23 amino acid residue hydrophobic signal peptide that is cleaved to yield an 8 kDa, 75 aa mature CCL13. Mature CCL13 lacks any potential N-glycosylation sites and shares a pyroglutamate proline motif with other human MCP proteins. Human CCL13 is most homologous to MCP-1, 3 and Eotaxin, exhibiting approximately 65-66% amino acid sequence identity. CCL13 mRNA is expressed by a number of activated cell types, including endothelial cells, macrophages, bronchial epithelium and type II alveolar cells, and perhaps lymphocytes. CCL13 is a chemoattractant for monocytes and eosinophils, and activates basophils. In addition, it has been reported to be chemotactic for CD4⁺ and CD8⁺ T cells, with an activity almost equivalent to that of MCP-3. The bioactivities of CCL13 is most likely mediated by the CC chemokine receptors CCR-2 and CCR-3, both of which have been shown to bind CCL13.

Entrez Gene IDs

6357 (Human)

Alternate Names

C-C motif chemokine 13; CCL13; chemokine (C-C motif) ligand 13; CKb10; MCP4; MCP-4; MCP-4Monocyte chemoattractant protein 4; MGC17134; NCC-1Monocyte chemotactic protein 4; NCC1Small-inducible cytokine A13; new CC chemokine 1; SCYA13CK-beta-10; SCYL1; small inducible cytokine subfamily A (Cys-Cys), member 13

Product Datasheets

Product Datasheet

COA

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Related Research Areas

Citations for Human CCL13/MCP-4 Antibody

R&D Systems personnel manually curate a database that contains references using R&D Systems products. The data collected includes not only links to publications in PubMed, but also provides information about sample types, species, and experimental conditions.

3 Citations: Showing 1 - 3
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CCL13 is a promising diagnostic marker for systemic sclerosis.
Authors: Yanaba K, Yoshizaki A, Muroi E, Hara T, Ogawa F, Shimizu K, Hasegawa M, Fujimoto M, Takehara K, Sato S
Br. J. Dermatol., 2009-09-11;162(2):332-6.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr
Common and differential chemokine expression patterns in rs cells of NLP, EBV positive and negative classical Hodgkin lymphomas.
Authors: Maggio EM, Van Den Berg A, Visser L, Diepstra A, Kluiver J, Emmens R, Poppema S
Int. J. Cancer, 2002-06-10;99(5):665-72.
Species: Human
Sample Types: Whole Cells, Whole Tissue
Applications: ICC, IHC
Sequential involvement of CCR2 and CCR6 ligands for immature dendritic cell recruitment: possible role at inflamed epithelial surfaces.
Authors: Vanbervliet B, Homey B, Durand I, Massacrier C, Ait-Yahia S, de Bouteiller O, Vicari A, Caux C
Eur J Immunol, 2002-01-01;32(1):231-42.
Species: Human
Sample Types: Whole Tissue
Applications: IHC-Fr