Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization

Cited:

Immunohistochemistry-Frozen, Immunocytochemistry, Antibody Array Development

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 52130
View all CCL15/MIP-1 delta Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human CCL15/MIP-1 delta
Gln22-Ile113
Accession # Q16663.2

Specificity

Detects human CCL15/MIP‑1 delta in ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Endotoxin Level

<0.10 EU per 1 μg of the antibody by the LAL method.

Scientific Data Images for Human CCL15/MIP-1 delta Antibody (52130)

Chemotaxis Induced by CCL15/MIP‑1 delta and Neutral-ization by Human CCL15/ MIP‑1 delta Antibody.

Chemotaxis Induced by CCL15/MIP‑1 delta and Neutral-ization by Human CCL15/ MIP‑1 delta Antibody.

Recombinant Human CCL15/ MIP-1d (Catalog # 363-MG) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR1 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL15/ MIP-1d (16 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL15/MIP-1d Mono-clonal Antibody (Catalog # MAB363). The ND50 is typically 3-15 µg/mL.
Human CCL15 / MIP-1 delta ELISA Standard Curve

Human CCL15 / MIP-1 delta ELISA Standard Curve

Human CCL15/MIP-1 delta was serially diluted and captured by Mouse Anti-Human CCL15/MIP‑1 delta Monoclonal Antibody (Catalog # MAB363) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL15/MIP‑1 delta Antigen Affinity-purified Polyclonal Antibody (Catalog # AF363) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)

Applications for Human CCL15/MIP-1 delta Antibody (52130)

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human CCL15/MIP‑1 delta 92 aa (Catalog # 363-MG)

Neutralization

Measured by its ability to neutralize CCL15/MIP‑1 delta -induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR1. The Neutralization Dose (ND50) is typically 3-15 µg/mL in the presence of 16 ng/mL Recombinant Human CCL15/MIP‑1 delta 92 aa.

Human CCL15/MIP-1 delta Sandwich Immunoassay

ELISA Capture (Matched Antibody Pair)
Recommended Concentration: 2-8 µg/mL
Use in combination with these reagents:
  • Detection Reagent: Human CCL15/MIP‑1 delta Biotinylated Antibody (Catalog # BAF363)
  • Standard: Recombinant Human CCL15/MIP-1 delta/LKN-1 (92 aa) Protein (Catalog # 363-MG)
Please Note: Optimal dilutions of this antibody should be experimentally determined.

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: CCL15/MIP-1 delta

CCL15, a human CC chemokine, was isolated from a human fetal spleen cDNA library. CCL15 cDNA encodes a predicted 113 amino acid (aa) protein containing a putative signal peptide of 21 aa that is cleaved to generate a 92 aa residue mature protein. Within the CC family members, human CCL15 shares 45%, 44%, 35%, and 30% aa homology with mouse C10, human MPIF-1, human HCC-1, and mouse MIP-1 gamma, respectively. The gene for MIP-1 delta is found on chromosome 17 where the genes for most of the human CC chemokines are located. Human CCL15 is expressed in T and B lymphocytes, NK cells, monocytes and monocyte-derived dendritic cells. Human MIP-1 delta is chemotactic for T cells and monocytes and has been shown to induce calcium flux in human CCR-1-transfected cells.

References

  1. Wang, W. et al. (1998) J. Clinical Immunol. 18:214

Alternate Names

HCC-2, Leukotactin 1, MIP-1 delta, MIP-5, MIP1 delta, NCC-3

Entrez Gene IDs

6359 (Human)

Gene Symbol

CCL15

UniProt

Q16663.2

Additional CCL15/MIP-1 delta Products

Product Documents for Human CCL15/MIP-1 delta Antibody (52130)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human CCL15/MIP-1 delta Antibody (52130)

For research use only

Citations for Human CCL15/MIP-1 delta Antibody (52130)

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