CCL22, also named stimulated T cell chemotactic protein (STCP-1), is a CC chemokine initially isolated from clones of monocyte-derived macrophages. Human CCL22 cDNA encodes a precursor protein of 93 amino acid residues with a 24 amino acid residue predicted signal peptide that is cleaved to yield a 69 amino acid residue mature 8 kDa protein. At the amino acid sequence level, CCL22 shows less than 35% identity to other CC chemokine family members. Human CCL22 is expressed in dendritic cells, macrophages and activated monocytes. In addition, CCL22 expression is also detected in the tissues of thymus, lymph node and appendix. The gene for human CCL22 has been mapped to chromosome 16 rather than chromosome 17 where the genes for many human CC chemokines are clustered. Recombinant or chemically synthesized mature CCL22 has been shown to induce chemotaxis or Ca2+ mobilization in dendritic cells, IL-2 activated NK cells, and activated T lymphocytes. A CD8+ T lymphocyte-derived secreted soluble activity that suppresses infection by primary non-syncytium-inducing and syncytium-inducing HIV-1 isolates and the T cell line-adapted isolate HIV-1IIIB, has been identified as CCL22. Based on amino-terminal sequence analysis, the major CD8+ T lymphocyte-derived CCL22 protein yielded an amino-terminal sequence of YGANM, which is two amino acid residues shorter than the predicted mature CCL22. The difference in potency between the two mature CCL22 isoforms has not been determined.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, Neutralization
Cited:
Neutralization, Flow Cytometry, Bioassay
Label
Unconjugated
Antibody Source
Polyclonal Chicken IgY
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Product Specifications
Immunogen
E. coli-derived recombinant human CCL22/MDC (R&D Systems, Catalog # 336-MD)
Gly25-Gln93
Accession # O00626
Gly25-Gln93
Accession # O00626
Specificity
Detects human CCL22/MDC in direct ELISAs and Western blots. In direct ELISAs, less than 5% cross-reactivity with recombinant mouse MDC and recombinant human MPIF-1 is observed.
Clonality
Polyclonal
Host
Chicken
Isotype
IgY
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL22/MDC Antibody
Chemotaxis Induced by CCL22/MDC and Neutralization by Human CCL22/MDC Antibody.
Recombinant Human CCL22/MDC (Catalog # 336-MD) chemoattracts the BaF3 mouse pro-B cell line transfected with human CCR4 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL22/MDC (0.03 µg/mL) is neutralized (green line) by increasing concentrations of Chicken Anti-Human CCL22/MDC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF336). The ND50 is typically 0.4-2.0 µg/mL.Applications for Human CCL22/MDC Antibody
Application
Recommended Usage
Western Blot
0.1 µg/mL
Sample: Recombinant Human CCL22/MDC (Catalog # 336-MD)
Sample: Recombinant Human CCL22/MDC (Catalog # 336-MD)
Neutralization
Measured by its ability to neutralize CCL22/MDC-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with human CCR4. The Neutralization Dose (ND50) is typically 0.4-2.0 µg/mL in the presence of 0.03 µg/mL Recombinant Human CCL22/MDC.
Reviewed Applications
Read 1 review rated 3 using AF336 in the following applications:
Formulation, Preparation, and Storage
Purification
Antigen Affinity-purified from egg yolks
Reconstitution
Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL22/MDC
Alternate Names
ABCD-1, MDC, STCP-1
Gene Symbol
CCL22
UniProt
Additional CCL22/MDC Products
Product Documents for Human CCL22/MDC Antibody
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL22/MDC Antibody
For research use only
Citations for Human CCL22/MDC Antibody
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Customer Reviews for Human CCL22/MDC Antibody (1)
3 out of 5
1 Customer Rating
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Application: ELISASample Tested: Serum and PlasmaSpecies: HumanVerified Customer | Posted 06/09/2020We used this antibody in an in-house ELISA along with mAb (336) and protein standard (336-MD) to quantify CCL22 in human serum and plasma. This combination could not detect CCL22 in our samples but generated a good standard curve.
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
Th2 Differentiation Pathway
