Eotaxin-2, also named MPIF-2 and Ck beta 6, is a CC chemokine that is designated CCL24. Eotaxin-2 cDNA encodes a 10.5 kDa, 119 amino acid residue precursor protein with a 26 aa residue signal peptide that is cleaved to generate a mature protein predicted to contain 93 amino acid residues with an N-glycosylation site. C‑terminally truncated variants with 78, 73, 75 and 76 residues have also been described. Eotaxin-2 shares 40%, 42% and 39% amino acid sequence identity with other CC chemokines CCL7/MCP-3, CCL3/MIP-1 alpha, and CCL11/Eotaxin, respectively. Eotaxin-2 mRNA is weakly expressed in activated monocytes and T lymphocytes. Recombinant Eotaxin-2 induces chemotaxis of eosinophils, basophils, and resting T lymphocytes, but not monocytes or activated T lymphocytes. Eotaxin-2 also suppresses colony formation by high proliferative multipotential hematopoietic progenitors. On eosinophils, the effects of Eotaxin-2, Eotaxin and CCL13/MCP-4 are mediated by the receptor CCR3.
Human CCL24/Eotaxin-2/MPIF-2 Antibody (61016)
R&D Systems | Catalog # MAB343
Scientific images may be resized, cropped, or adjusted for brightness or contrast for presentation purposes. These updates are limited to presentation and do not affect the underlying scientific interpretation of the data. Where available, additional source data may be provided upon request.
Key Product Details
Species Reactivity
Validated:
Human
Cited:
Human
Applications
Validated:
Western Blot, ELISA Capture (Matched Antibody Pair), Neutralization
Cited:
ELISA Development
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG1 Clone # 61016
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Product Specifications
Immunogen
E. coli-derived recombinant human CCL24/Eotaxin‑2/MPIF‑2
Val27-Cys119
Accession # AAB51135
Val27-Cys119
Accession # AAB51135
Specificity
Detects human CCL24/Eotaxin‑2/MPIF‑2 in ELISAs and Western blots. In sandwich ELISAs, less than 0.04% cross-reactivity with recombinant human (rh) Eotaxin, rhMIP-1 alpha, and rhMCP-3 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG1
Endotoxin Level
<0.10 EU per 1 μg of the antibody by the LAL method.
Scientific Data Images for Human CCL24/Eotaxin-2/MPIF-2 Antibody (61016)
Chemotaxis Induced by CCL24/Eotaxin‑2 and Neutralization by Human CCL24/Eotaxin‑2 Antibody.
Recombinant Human CCL24/ Eotaxin-2 (Catalog # 343-E2) chemoattracts the BaF3 mouse pro-B cell line transfected with mouse CCR3 in a dose-dependent manner (orange line). The amount of cells that migrated through to the lower chemotaxis chamber was measured by Resazurin (Catalog # AR002). Chemotaxis elicited by Recombinant Human CCL24/ Eotaxin-2 (100 ng/mL) is neutralized (green line) by increasing concentrations of Mouse Anti-Human CCL24/ Eotaxin-2 Monoclonal Antibody (Catalog # MAB343). The ND50 is typically 0.2-0.8 µg/mL.
Human CCL24 / Eotaxin-2 / MPIF-2 ELISA Standard Curve
Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2) was serially diluted and captured by Mouse Anti-Human CCL24/Eotaxin‑2/MPIF‑2 Monoclonal Antibody (Catalog # MAB343) coated on a Clear Polystyrene Microplate (Catalog # DY990). Goat Anti-Human CCL24/Eotaxin‑2/MPIF‑2 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF343) was biotinylated and incubated with the protein captured on the plate. Detection of the standard curve was achieved by incubating Streptavidin-HRP (Catalog # DY998)Applications for Human CCL24/Eotaxin-2/MPIF-2 Antibody (61016)
Application
Recommended Usage
Western Blot
1 µg/mL
Sample: Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2)
Sample: Recombinant Human CCL24/Eotaxin‑2/MPIF‑2 (Catalog # 343-E2)
Neutralization
Measured by its ability to neutralize CCL24/Eotaxin‑2/MPIF‑2-induced chemotaxis in the BaF3 mouse pro‑B cell line transfected with mouse CCR3. The Neutralization Dose (ND50) is typically 0.2-0.8 µg/mL in the presence of 100 ng/mL Recombinant Human CCL24/Eotaxin‑2/MPIF‑2.
Human CCL24/Eotaxin-2/MPIF-2 Sandwich Immunoassay
Please Note: Optimal dilutions of this antibody should be experimentally determined.
Formulation, Preparation, and Storage
Purification
Protein A or G purified from ascites
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CCL24/Eotaxin-2/MPIF-2
References
- Forssmann, U. et al. (1997) J. Exp. Med. 185:2171.
- Patel, V.P. et al. (1997) J. Exp. Med. 185:1163.
Alternate Names
Eotaxin-2, MPIF-2, MPIF2
Gene Symbol
CCL24
UniProt
Additional CCL24/Eotaxin-2/MPIF-2 Products
Product Documents for Human CCL24/Eotaxin-2/MPIF-2 Antibody (61016)
Certificate of Analysis
To download a Certificate of Analysis, please enter a lot or batch number in the search box below.
Note: Certificate of Analysis not available for kit components.
Product Specific Notices for Human CCL24/Eotaxin-2/MPIF-2 Antibody (61016)
For research use only
Related Research Areas
Citations for Human CCL24/Eotaxin-2/MPIF-2 Antibody (61016)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars
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Associated Pathways
