Cyclin dependent kinase 5 regulatory subunit 1 (CDK5R1) is a 35 kDa protein that associates with and activates CDK5. Its expression is restricted to neurons and is required for normal brain development. CDK5R1 can be cleaved by calpain at Phe98-Ala99 which liberates a C-terminal fragment known as p25. p25 induces prolonged activation of CDK5 and broadens the substrate specificity of CDK5 to include tau which is aberrantly phosphorylated in Alzheimer's disease. Full length CDK5R1 is myristoylated at Gly2 and is preferentially localized to membranes at the cell periphery. p25 lacks this modification and is enriched in the nucleus and perinuclear regions. p25 accumulates in the brains of Alzheimer's patients where it promotes neuronal apoptosis and cytoskeletal abnormalities. Within aa 100-307, human CDK5R1 shares 99% aa sequence identity with mouse and rat CDK5R1.
Human CDK5 Activator 1 Antibody (739304)
R&D Systems | Catalog # MAB7357
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Key Product Details
Species Reactivity
Applications
Label
Antibody Source
Product Specifications
Immunogen
Ser8-Phe98
Accession # Q15078
Specificity
Clonality
Host
Isotype
Scientific Data Images for Human CDK5 Activator 1 Antibody (739304)
Detection of Human CDK5 Activator 1 by Western Blot.
Western blot shows lysates of human brain (cortex) tissue and human brain (hippocampus) tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human CDK5 Activator 1 Monoclonal Antibody (Catalog # MAB7357) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for CDK5 Activator 1 at approximately 35 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.Applications for Human CDK5 Activator 1 Antibody (739304)
Western Blot
Sample: Human brain (cortex) tissue and human brain (hippocampus) tissue
Reviewed Applications
Read 1 review rated 4 using MAB7357 in the following applications:
Formulation, Preparation, and Storage
Purification
Reconstitution
Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.
Formulation
Shipping
Stability & Storage
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: CDK5 Activator 1
Long Name
Alternate Names
Gene Symbol
UniProt
Additional CDK5 Activator 1 Products
Product Documents for Human CDK5 Activator 1 Antibody (739304)
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Product Specific Notices for Human CDK5 Activator 1 Antibody (739304)
For research use only
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Citations for Human CDK5 Activator 1 Antibody (739304)
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
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