Human CEBP epsilon Alexa Fluor® 647-conjugated Antibody

Catalog #: FAB6726R Datasheet / COA / SDS

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FAB6726R-100UG

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Human CEBP epsilon Alexa Fluor® 647-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CEBP epsilon in direct ELISAs and Western blots. In direct ELISAs and Western blots, no cross-reactivity with recombinant human CEBP alpha, beta, delta, gamma, or zeta is observed.

Source

Monoclonal Mouse IgG1 Clone # 695345

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human CEBP Îµ
Thr151-Arg210
Accession # Q15744

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 647 (Excitation= 650 nm, Emission= 668 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

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Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CEBP epsilon

CCAAT-enhancer binding protein epsilon (CEBP epsilon ) is a 32 kDa basic-leucine zipper transcription factor that is expressed in myeloblasts, granulocytes, and eosinophils. Multiple isoforms of human CEBP epsilon are generated by alternate splicing and alternative translation initiation sites. All four isoforms contain the bZIP domain (aa 208-267) but exert different effects on myeloid differentiation. Full length CEBP epsilon and the 30 kDa isoform (aa 33-281) cooperate with c-Myb to activate the transcription of genes involved in myeloid differentiation. The 27 kDa isoform (which lacks aa 69-97) and the 14 kDa isoform (which begins at Met153) function as transcriptional repressors in eosinophils. Within aa 151-210, human CEBP epsilon shares 93% amino acid sequence identity with mouse and rat CEBP epsilon, respectively.

Long Name

CCAAT/enhancer-Binding Protein epsilon

Entrez Gene IDs

1053 (Human); 110794 (Mouse); 25410 (Rat)

Alternate Names

C/EBP-epsilon; CCAAT/enhancer binding protein (C/EBP), epsilon; CCAAT/enhancer-binding protein epsilon; CEBP epsilon; CEBPE; CRP1C/EBP epsilon