Human Chitinase 3-like 2 Antibody (632010)

R&D Systems | Catalog # MAB5116

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot

Cited:

Immunohistochemistry, Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 632010
View all Chitinase 3-like 2 Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Chitinase 3-like 2
Tyr27-Leu390
Accession # Q15782

Specificity

Detects human Chitinase 3-like 2 in direct ELISAs and Western blots. In direct ELISAs, no cross-reactivity with recombinant human (rh) Chitinase 3‑like 1 or rhChitotriosidase is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human Chitinase 3-like 2 Antibody (632010)

Detection of Human Chitinase 3-like 2 antibody by Western Blot.

Detection of Human Chitinase 3-like 2 by Western Blot.

Western blot shows lysates of human lung tissue. PVDF Membrane was probed with 2 µg/mL of Mouse Anti-Human Chitinase 3-like 2 Monoclonal Antibody (Catalog # MAB5116) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Chitinase 3-like 2 at approximately 40 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Detection of Chitinase 3-like 2 by Western Blot

Detection of Chitinase 3-like 2 by Western Blot

Breast cancer cells models with different status of CHI3L2 after transfection with CHI3L2 siRNA: (A) changing in STAT-3 and ERK1/2 phosphorylation; Western blot analysis; (B) analysis of CHI3L2 mRNA in MDA-MB-321 and (C) BT-549 cells during trnasfection experiments provided in triplicates in ddPCR analysis; and (D) with presence of recombinant CHI3L2 protein in MDA-MB-468 and BT474 cells ; Western blot analysis; Kruskal–Wallis test followed by Dunn test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39557919), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Chitinase 3-like 2 by Western Blot

Detection of Chitinase 3-like 2 by Western Blot

Western blot analysis of CHI3L2 expression in IDC in A tumors of different malignancy grade: G1 (n = 3), G2 (n = 9) and G3 (n = 10), with expressions of pSTAT-3, STAT-3, pERK1/2 and ERK1/2; B statistical analysis (data from densitometric analysis of Western blots experiments provided in triplicates) on CHI3L2, pSTAT-3 and pERK1/2 expressions in G1-G3 grade tumors (Kruskal–Wallis test, post-hoc Dunn’s test). Raw data from densitrometric analysis is showed in Fig. 2S supplemental material. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39557919), licensed under a CC-BY license. Not internally tested by R&D Systems.
Detection of Chitinase 3-like 2 by Western Blot

Detection of Chitinase 3-like 2 by Western Blot

Breast cancer cells models with different status of CHI3L2 after transfection with CHI3L2 siRNA: (A) changing in STAT-3 and ERK1/2 phosphorylation; Western blot analysis; (B) analysis of CHI3L2 mRNA in MDA-MB-321 and (C) BT-549 cells during trnasfection experiments provided in triplicates in ddPCR analysis; and (D) with presence of recombinant CHI3L2 protein in MDA-MB-468 and BT474 cells ; Western blot analysis; Kruskal–Wallis test followed by Dunn test Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/39557919), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Chitinase 3-like 2 Antibody (632010)

Application
Recommended Usage

Western Blot

2 µg/mL
Sample: Human lung tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Chitinase 3-like 2

Chitinase 3-like 2 (CHI3L2; also chrondrocyte protein 39 and YKL-39) is a 39 kDa glycoprotein member of the glycosyl hydrolase 18 family. It is secreted by synovial fibroblasts and chondrocytes, particularly in osteoarthritis, and may contribute to extracellular matrix stability. CHI3L2 has no chitotriosidase activity, but is likely to bind some type of glycan. The human CHI3L2 precursor is 390 amino acids (aa) in length. It contains a 26 aa signal sequence and a 364 aa mature region. There are two potential alternate start sites at Met24 and Met80; a third potential isoform shows a deletion of aa 15‑24. Over aa 27‑390, human CHI3L2 shares 89% aa identity with bovine CHI3L2. No rodent CHI3L2 has been reported.

Alternate Names

CHI3L2, Chitinase 3 like 2, YKL39

Entrez Gene IDs

1117 (Human)

Gene Symbol

CHI3L2

UniProt

Q15782 (Human)

Additional Chitinase 3-like 2 Products

Product Documents for Human Chitinase 3-like 2 Antibody (632010)

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Product Specific Notices for Human Chitinase 3-like 2 Antibody (632010)

For research use only

Citations for Human Chitinase 3-like 2 Antibody (632010)

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