Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Complement Component C1qC Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human C1qC
Lys115-Asp245
Accession # P02747

Specificity

Detects human Complement C1qC in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 1% cross-reactivity with recombinant human (rh) C1qA and rhC1qB is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Complement Component C1qC Antibody

Complement Component C1qC antibody in Human HUVEC by Immunocytochemistry (ICC).

Complement Component C1qC in Human HUVEC.

Complement Component C1qC was detected in immersion fixed HUVEC human umbilical vein endothelial cells using Goat Anti-Human Complement Component C1qC Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3337) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Applications for Human Complement Component C1qC Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed HUVEC human umbilical vein endothelial cells

Western Blot

0.1 µg/mL
Sample: Recombinant Human Complement Component C1qC

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Complement Component C1qC

C1qC is a globular secreted protein and is a subunit of complement component C1q. C1q is composed of 18 polypeptide chains of three distinct subtypes (C1qA, C1qB and C1qC) arranged as 6 disulfide-linked A-B heterodimers and 3 disulfide-linked C-C homodomers. All C1q subunits contain a C1q globular domain and one collagen-like domain. C1q is a subcomponent of complement C1 complex which comprises one C1q and the Ca2+-dependent serine protease tetramer C1r2-C1s2. C1 complex triggers the classical complement pathway. Within the C1q domain, human C1qC shares approximately 75% amino acid sequence identity with mouse and rat C1qC.

Long Name

Complement Component 1q Subcomponent Subunit C Precursor

Alternate Names

C1qC

Entrez Gene IDs

714 (Human)

Gene Symbol

C1QC

UniProt

P02747

Additional Complement Component C1qC Products

Product Documents for Human Complement Component C1qC Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Complement Component C1qC Antibody

For research use only

Citations for Human Complement Component C1qC Antibody

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Protocols

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