Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunoprecipitation

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 269716
View all Complement Component C2b Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Complement Component C2
Ala21-Leu752
Accession # P06681

Specificity

Detects human Complement Component C2b in direct ELISAs and Western blots. In direct ELISAs, no cross‑reactivity with recombinant human (rh) C1r, rhC1s, rhC3r, rhC5a, rhFactor B, or rhFactor I is observed. In Western blots, this antibody recognizes rhC2 and rhC2B.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human Complement Component C2b Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human Complement Component C2b, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human Complement Component C2b
under non-reducing conditions only

Reviewed Applications

Read 4 reviews rated 4.3 using MAB1936 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

=
÷

Background: Complement Component C2b

C2 is a key protease involved in the classical pathway of complement. It is synthesized as a single-chain and is proteolytically processed into two chains, C2a (aa 244‑752) and C2b (aa 21‑243). C2a consists of a vWF domain and a serine protease domain. C2b contains 3 Sushi (SCR) domains.

Alternate Names

ARMD14, CO2

Entrez Gene IDs

717 (Human); 12263 (Mouse); 24231 (Rat)

Gene Symbol

C2

UniProt

P06681

Additional Complement Component C2b Products

Product Documents for Human Complement Component C2b Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human Complement Component C2b Antibody

For research use only

Citations for Human Complement Component C2b Antibody

Customer Reviews for Human Complement Component C2b Antibody (4)

4.3 out of 5
4 Customer Ratings
5 Stars
25%
4 Stars
75%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human Complement Component C2b Antibody?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 4 的 4 reviews Showing All
Filter By:
Clear Filters X
  • Human Complement Component C2b Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 05/12/2021
    This antibody was used to coat the plate for a C2b immunoassay.
    Human Complement Component C2b Antibody MAB1936
  • Human Complement Component C2b Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Complement Component C2b Antibody MAB1936
  • Human Complement Component C2b Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/07/2020
    Antibody was printed on custom arrays and incubated with fluorescently labeled human EDTA plasma
    Human Complement Component C2b Antibody MAB1936
  • Human Complement Component C2b Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018

There are no reviews that match your criteria.

Showing  1 - 4 的 4 reviews Showing All

FAQs

No product specific FAQs exist for this product.

View all FAQs for Antibodies