Human CSL Alexa Fluor® 750-conjugated Antibody

Catalog #: AF4079S Datasheet / COA / SDS

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AF4079S-100UG

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Human CSL Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human CSL in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human CSL
Gly208-Ser500
Accession # Q06330

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: CSL

CSL (CBF1/RBP-Jk; Su(H)/Suppressor of Hairless; Lag-1) is a 56 kDa member of the Su(H) family of transcription factors. It is constitutively bound to DNA where it participates in gene repression. Upon ligand binding, the intracellular domain of Notch translocates to the nucleus and interacts with CSL, allowing transcription of Notch target genes. Human CSL is 500 amino acids (aa) in length. It contains three DNA binding sites (aa 58‑65; 192‑201; and 265‑297) and one IPT domain (aa 355‑445). Multiple splice variants exist that involve the first 89 N-terminal amino acids. One variant (isoform 2) is 487 aa in length and shows a 6 aa substitution for amino acids 1‑19. Over amino acids 195‑487, human CSL isoform 2 shares more than 98% aa sequence identity with mouse, rat, and canine CSL.

Long Name

Recombining Binding Protein Supressor of Hairless [CBF1, CSL]

Entrez Gene IDs

3516 (Human); 19664 (Mouse)

Alternate Names

CBF1; CBF-1; CSL; H-2K binding factor-2; IGKJRB1; IGKJRB1RBPSUHCBF1; IGKJRBRBP-J kappa; immunoglobulin kappa J region recombination signal binding protein 1; J kappa-recombination signal-binding protein; KBF2; RBPJ; RBP-JK; RBPJKMGC61669; RBP-Jrecombining binding protein suppressor of hairless (Drosophila); RBPSUH; recombination signal binding protein for immunoglobulin kappa J region; recombining binding protein suppressor of hairless; Renal carcinoma antigen NY-REN-30; SUH; suppressor of hairless homolog