Human Cyclin E1 Antibody

R&D Systems | Catalog # MAB68101

R&D Systems
Discontinued Product
MAB68101 has been discontinued. An alternative/replacement product is available: AF6810. View all Cyclin E1 products.

Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 713548
View all Cyclin E1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Cyclin E1
Met16-Lys143
Accession # P24864

Specificity

Detects human Cyclin E1 in direct ELISAs and Western blots.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Cyclin E1 Antibody

Detection of Human Cyclin E1 antibody by Western Blot.

Detection of Human Cyclin E1 by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Cyclin E1 Monoclonal Antibody (Catalog # MAB68101) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Cyclin E1 at approximately 65 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human Cyclin E1 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and K562 human chronic myelogenous leukemia cell line

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Cyclin E1

Cyclin E1 (also known as G1/S-specific cyclin-E1 and CCNE1) is a 48-58 kDa member of the cyclin E subfamily, cyclin family of molecules. It associates with Cdk2 kinase, and determines substrate specificity for the complex. This complex phosphorylates multiple substrates involved in cell cycle progression and the initiation of DNA replication. Cyclin E1 is required for G1/S phase progression and cell cycle reentry from G0 phase. Its reduced activity during cellular senescence contributes to G1 arrest. Human cyclin E1 is 410 amino acids (aa) in length. It contains two cyclin box folds (aa 144-234 and 293-363) and is phosphorylated on at least eight Ser/Thr sites. There are at least two alternate splice forms. One is 40-41 kDa in size and utilizes an alternate start site at Met46, while a second is 43-44 kDa in size and shows a deletion of aa 154-196. One other potential start site exists at Met16. There are multiple proteolytic cleavage products that are tumor-associated and show increased destabilizing activity. Cleavage around Gln40 generates 44-45 kDa C-terminal fragments, while cleavage between Ala69Asp70 generates 33-35 kDa C-terminal fragments. Over aa 16-143, human cyclin E1 shares 67% aa identity with mouse cyclin E1.

Alternate Names

CCNE, CCNE1

Entrez Gene IDs

898 (Human); 12447 (Mouse); 25729 (Rat)

Gene Symbol

CCNE1

UniProt

P24864 (Human)

Additional Cyclin E1 Products

Product Documents for Human Cyclin E1 Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Cyclin E1 Antibody

For research use only

Related Research Areas

Cell Cycle

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