Human DEC-205/CD205 Antibody

Name: Human DEC-205/CD205 Antibody
Brand: R&D Systems
SKU: AF2047
Rating: 4 (1 reviews)

Catalog #: AF2047 1 Review Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
AF2047
AF2047-SP

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Detection of Human DEC‑205/CD205 by Western Blot.

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Human DEC-205/CD205 Antibody Summary

Species Reactivity

Human

Specificity

Detects human DEC‑205/CD205 in direct ELISAs and Western blots.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DEC‑205/CD205
Cys216-Cys501
Accession # O60449

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

1 µg/mL

See below

Immunocytochemistry

5-15 µg/mL

See below

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Human DEC‑205/CD205 by Western Blot. Western blot shows lysates of human thymus tissue. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2047) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF017). A specific band was detected for DEC-205/ CD205 at approximately 250 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Immunocytochemistry

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DEC‑205/CD205 in Human PBMCs. DEC-205/CD205 was detected in immersion fixed human peripheral blood mononuclear cells (PBMCs) using 10 µg/mL Goat Anti-Human DEC-205/CD205 Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2047) for 3 hours at room temperature. Cells were stained with the Northern-Lights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Non-adherent Cells.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: DEC-205/CD205

DEC-205, also known as CD205 and lymphocyte antigen 75 (Ly 75), is a type I transmembrane protein that is primarily expressed on dendritic cells and thymic epithelial cells. The extracellular region of DEC-205 contains ten C-type lectin-like domains, a fibronectin type II domain and a ricin B-type lectin domain. DEC-205 functions as an endocytic receptor for antigens. The recombinant protein used to generate the anti‑human DEC-205 antibody contains the first two C-type lectin domains.

Entrez Gene IDs

4065 (Human); 17076 (Mouse)

Alternate Names

CD205; CLEC13B; C-type lectin domain family 13 member B; DEC205; DEC-205; GP200-MR6; Ly75; Ly-75; Lymphocyte antigen 75