Human DFF40/CAD Alexa Fluor® 488-conjugated Antibody

Catalog #: FAB6289G Datasheet / COA / SDS

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FAB6289G-100UG

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All DFF40/CAD Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DFF40/CAD Alexa Fluor® 488-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DFF40/CAD in direct ELISAs and Western blots.

Source

Monoclonal Mouse IgG1 Clone # 609809

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human DFF40/CAD
Met1-Gln338
Accession # O76075

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 488 (Excitation= 488 nm, Emission= 515-545 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DFF40/CAD

DNA fragmentatation factor 40 kDa subunit (DFF40), also known as CAD, is a member of the CIDE domain-containing proteins. It is an apoptotic nuclease that is maintained in an inactive state by association with DFF45. Apoptotic signaling induces DFF45 cleavage and the release of DFF40. Active DFF40 exists as an oligomer and generates double stranded breaks in dsDNA. Alternate splicing of human DFF40 generates isoforms with substitutions and truncations either directly following the CIDE domain (which is aa 4‑80) or at the C-terminus. Full length human DFF40 (aa 1‑338) shares 76% and 74% aa sequence identity with mouse and rat DFF40, respectively.

Long Name

DNA Fragmentation Factor, Subunit beta

Entrez Gene IDs

1677 (Human); 13368 (Mouse); 84359 (Rat)

Alternate Names

CAD; CADCaspase-activated nuclease; Caspase-activated deoxyribonuclease; Caspase-activated DNase; CPAN; CPANDNA fragmentation factor 40 kDa subunit; DFF2; DFF40; DFF-40DFF40DFF2DNA fragmentation factor subunit beta; DFFB; DNA fragmentation factor, 40kDa, beta polypeptide (caspase-activated DNase); EC 3.-