Key Product Details

Species Reactivity

Human

Applications

Western Blot

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 378703
View all DLL3 Primary Antibodies »

Product Specifications

Immunogen

Chinese hamster ovary cell line CHO-derived recombinant human DLL3
Ala27-Pro488
Accession # Q9NYJ7

Specificity

Detects human DLL3 in direct ELISAs and Western blots. Does not cross‑react with recombinant human (rh) DLL1 or rhDLL4.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Applications for Human DLL3 Antibody

Application
Recommended Usage

Western Blot

1 µg/mL
Sample: Recombinant Human DLL3

Reviewed Applications

Read 8 reviews rated 4.3 using MAB4315 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DLL3

Delta-like 3 (DLL3) is a transmembrane Delta-like protein that contains one DSL domain and six EGF-like repeats. DLL3 is critical for somite segmentation and neurogenesis during early embryonic development. It does not bind Notch receptors but functions as an antagonist of Notch signaling. Defects in DLL3 cause axial skeletal abnormalities in spondylocostal dysostosis. Within the extracellular domain, human DLL3 shares 84% amino acid sequence identity with mouse and rat DLL3.

Long Name

Delta-like 3

Alternate Names

Pudgy, SCDO1

Entrez Gene IDs

10683 (Human); 13389 (Mouse); 114125 (Rat)

Gene Symbol

DLL3

UniProt

Q9NYJ7 (Human)

Additional DLL3 Products

Product Documents for Human DLL3 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DLL3 Antibody

For research use only

Related Research Areas

Cancer Biomarkers
Notch Pathway

Citations for Human DLL3 Antibody

Customer Reviews for Human DLL3 Antibody (8)

4.3 out of 5
8 Customer Ratings
5 Stars
50%
4 Stars
25%
3 Stars
25%
2 Stars
0%
1 Stars
0%

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Customer Images

Showing  1 - 5 的 8 reviews Showing All
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  • Human DLL3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 12/08/2021
    Human DLL3 Antibody MAB4315
  • Human DLL3 Antibody
    Name: Anonymous
    Application: Flow Cytometry
    Sample Tested: NCI-H82 lung cancer cell line
    Species: Human
    Verified Customer | Posted 12/23/2020
    Detection of DLL3 on lung cancer cell line NCI-H82. NCI-H82 cells were treated with 100 nM of the human DLL3 antibody (catalog # MAB4315) or mouse IgG2b isotype control, followed by a secondary antibody goat a-mouse IgG Fc APC. Isotype control (RED), human DLL3 antibody (BLUE).
    Human DLL3 Antibody MAB4315
  • Human DLL3 Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/03/2020
    Human DLL3 Antibody MAB4315
  • Human DLL3 Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 02/21/2019
  • Name: Jignesh Ganeshbhai Patel
    Application: Western Blot
    Sample Tested: Blood mononuclear cells (PBMCs)
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Immunocytochemistry/Immunofluorescence
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 09/10/2018
  • Name: Jignesh Patel
    Application: Western Blot
    Sample Tested: THP-1 human acute monocytic leukemia cell line
    Species: Human
    Verified Customer | Posted 08/21/2018
  • Human DLL3 Antibody
    Name: Anonymous
    Application: Western Blot
    Sample Tested: HEK293 human embryonic kidney cell line
    Species: Human
    Verified Customer | Posted 04/07/2016
    Recombinant DLL3 ECD Fc fusion was expressed transiently in 293. Generated conditioned media was run over Protein A column, then desalted, then polished on SEC column. The picture shown is a Western Blot for the collected SEC fractions, using MAB4315 as the primary antibody, followed by an anti-murine secondary antibody.
    Human DLL3 Antibody MAB4315

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