Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Immunohistochemistry-Paraffin

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all DNER Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human DNER
Arg29-His637
Accession # Q8NFT8

Specificity

Detects human DNER in direct ELISAs and Western blots. In direct ELISAs and Western blots, approximately 30% cross-reactivity with recombinant mouse DNER is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human DNER Antibody

Detection of Human DNER antibody by Western Blot.

Detection of Human DNER by Western Blot.

Western blot shows lysates of human brain (cerebellum) tissue. PVDF membrane was probed with 0.2 µg/mL of Goat Anti-Human DNER Antigen Affinity-purified Polyclonal Antibody (Catalog # AF3646) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF109). A specific band was detected for DNER at approximately 140 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human DNER Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed A172 human glioblastoma cell line

Western Blot

0.2 µg/mL
Sample: Human brain (cerebellum) tissue

Reviewed Applications

Read 1 review rated 5 using AF3646 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: DNER

DNER, also known as brain EGF repeat containing transmembrane protein (BET), is a neuron-specific type I transmembrane notch ligand that is expressed on membranes of dendrites and cell bodies but not axons. It contains ten extracellular EGF-like domains and one follistatin-like domain (between the 9th and 10th EGF-like domains). DNER mediates neuron-glia interactions and is required for the functional and morphological maturation of the cerebellum. The extracellular domains of human and mouse DNER share 89% amino acid sequence homology.

Long Name

Delta/Notch-like EGF Repeat-containing Transmembrane

Alternate Names

BET, delta and Notch-like epidermal growth factor-related receptor, delta/notch-like EGF repeat containing, delta-notch-like EGF repeat-containing transmembrane, H_NH0150O02.1, UNQ26, WUGSC:H_NH0150O02.1

Entrez Gene IDs

92737 (Human); 227325 (Mouse)

Gene Symbol

DNER

UniProt

Q8NFT8

Additional DNER Products

Product Documents for Human DNER Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Download SDS

Product Specific Notices for Human DNER Antibody

For research use only

Related Research Areas

Notch Pathway

Citations for Human DNER Antibody

Customer Reviews for Human DNER Antibody (1)

5 out of 5
1 Customer Rating
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  • Name: Anonymous
    Application: Immunofluorescence
    Sample Tested: HEK 293 cells
    Species: Human
    Verified Customer | Posted 01/13/2015

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Protocols

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FAQs

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Associated Pathways

Notch Signaling Pathways Notch Signaling Pathway Thumbnail