Human Dopa Decarboxylase/DDC Antibody

Catalog #: MAB3564 Datasheet / COA / SDS

Catalog #	Availability	Size / Price	Qty
MAB3564
MAB3564-SP

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Detection of Dopa Decarboxylase/DDC Mouse by Western Blot.

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Summary Data Examples Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human Dopa Decarboxylase/DDC Antibody Summary

Species Reactivity

Human

Source

Monoclonal Mouse IgG_2B Clone # 413911

Purification

Protein A or G purified from hybridoma culture supernatant

Immunogen

S. frugiperda insect ovarian cell line Sf 21-derived recombinant human DDC
Met1-Glu480
Accession # P20711

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Label

Unconjugated

Applications

Recommended Concentration

Sample

Western Blot

2 µg/mL

See below

Knockout Validated

Dopa Decarboxylase/DDC is specifically detected in Jurkat human acute T cell leukemia parental cell line but is not detectable in Dopa Decarboxylase/DDC knockout Jurkat cell line.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Scientific Data

Western Blot

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Detection of Dopa Decarboxylase/DDC Mouse by Western Blot. Western blot shows lysates of Jurkat human acute T cell leukemia cell line. PVDF membrane was probed with 2 µg/mL of Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF007). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Knockout Validated

Western Blot Shows Human Dopa Decarboxylase/DDC Antibody Specificity by Using Knockout Cell Line.

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Western Blot Shows Human Dopa Decarboxylase/DDC Specificity by Using Knockout Cell Line. Western blot shows lysates of Jurkat human acute T cell leukemia parental cell line and Dopa Decarboxylase/DDC Jurkat cell line (KO). PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human Dopa Decarboxylase/DDC Monoclonal Antibody (Catalog # MAB3564) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Dopa Decarboxylase/DDC at approximately 54 kDa (as indicated) in the parental Jurkat cell line, but is not detectable in knockout Jurkat cell line. GAPDH (Catalog # MAB5718) is shown as a loading control. This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Reconstitution Calculator

Preparation and Storage

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.

12 months from date of receipt, -20 to -70 °C as supplied.
1 month, 2 to 8 °C under sterile conditions after reconstitution.
6 months, -20 to -70 °C under sterile conditions after reconstitution.

Background: Dopa Decarboxylase/DDC

Dopa decarboxylase (DDC), also known as aromatic amino acid decarboxylase, is a group II decarboxylase (1). The enzyme catalyzes the decarboxylation of aromatic L-amino acids to produce the corresponding amines. DDC produces the neurotransmitters dopamine and serotonin from L-Dopa and L-5-hydroxytryptophan, respectively. The inhibition of DDC could be used for the treatment of schizophrenia and Parkinson's disease (2).

References

Sandmeier, E. et al. (1994) Eur. J. Biochem. 221:997.
Bertoldi, M. et al. (1996) J. Biol. Chem. 271:23954.

Entrez Gene IDs

1644 (Human)

Alternate Names

AADC; AADCaromatic-L-amino-acid decarboxylase; DDC; dopa decarboxylase (aromatic L-amino acid decarboxylase); Dopa Decarboxylase; EC 4.1.1; EC 4.1.1.28