Human DPPA2 Alexa Fluor® 532-conjugated Antibody

Catalog #: AF7086X Datasheet / COA / SDS

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AF7086X-100UG

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPPA2 Alexa Fluor® 532-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DPPA2 in direct ELISAs and Western blots.

Source

Polyclonal Sheep IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DPPA2
Ser2-Thr174
Accession # Q7Z7J5

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 532 (Excitation= 534 nm, Emission= 553 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DPPA2

DPPA2 (Developmental pluripotency associated 2; also ESCA, CT100 and PESCRG1) is a 38-39 kDa member of both the DPPA and cancer-testis family of proteins. It is found in pluripotent stem cells in a pattern that parallels that of Oct4, and likely contributes to formation of the ICM, plus the maintainence of cells in an undifferentiated state. DPPA2 is also found in spermatocytes and select tumor types. Human DPPA2 is 298 amino acids (aa) in length. It contains one DNA-binding SAP domain that is involved in chromosomal organization (aa 92-126). One potential isoform variant has been reported that shows a deletion of aa 61-71. Over aa 2‑174, human DPPA2 shares only 36% aa identity with mouse DPPA2.

Long Name

Developmental Pluripotency Associated Protein 2

Entrez Gene IDs

151871 (Human); 100043167 (Mouse); 685364 (Rat)

Alternate Names

CT100; developmental pluripotency associated 2; developmental pluripotency-associated protein 2; DPPA2; ECSA; PESCRG1; PESCRG1cancer/testis antigen 100; Pluripotent embryonic stem cell-related gene 1 protein