Human DPPA5/ESG1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF3125S Datasheet / COA / SDS

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AF3125S-100UG

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All DPPA5/ESG1 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human DPPA5/ESG1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human DPPA5/ESG1 in direct ELISAs and Western blots. In Western blots, approximately 40% cross-reactivity with recombinant mouse DPPA5 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human DPPA5/ESG1
Met1-Lys116
Accession # NP_001020461

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: DPPA5/ESG1

DPPA5, also known as embryonic stem cell-specific gene 1 (ESG1), is a K homology RNA-binding (KH) domain containing protein that is expressed only in early embryos, germ cells and embryonic stem cells. It may be useful as a marker of pluripotent stem cells. Human DPPA5 shares approximately 66% and 74% amino acid sequence homology with mouse and rat DPPA5, respectively.

Long Name

Developmental Pluripotency Associated 5

Entrez Gene IDs

340168 (Human); 434423 (Mouse); 301101 (Rat)

Alternate Names

developmental pluripotency associated 5; developmental pluripotency-associated 5 protein; DPPA5; embryonal stem cell specific gene 1; Embryonal stem cell-specific gene 1 protein; ESG1; ESG1ESG-1; hDPPA5