Key Product Details
Species Reactivity
Human
Applications
Western Blot, Immunoprecipitation
Label
Unconjugated
Antibody Source
Monoclonal Mouse IgG2A Clone # 500915
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Product Specifications
Immunogen
Mouse myeloma cell line NS0-derived recombinant human ENPP-5
Pro25-Ser430
Accession # Q9UJA9
Pro25-Ser430
Accession # Q9UJA9
Specificity
Detects human ENPP-5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-7, or recombinant mouse ENPP-7 is observed.
Clonality
Monoclonal
Host
Mouse
Isotype
IgG2A
Applications for Human ENPP‑5 Antibody
Application
Recommended Usage
Immunoprecipitation
25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ENPP-5, see our available Western blot detection antibodies
Sample: Conditioned cell culture medium spiked with Recombinant Human ENPP-5, see our available Western blot detection antibodies
Western Blot
1 µg/mL
Sample: Recombinant Human ENPP-5
Sample: Recombinant Human ENPP-5
Formulation, Preparation, and Storage
Purification
Protein A or G purified from hybridoma culture supernatant
Reconstitution
Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.
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Formulation
Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.
Shipping
Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.
Stability & Storage
Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
- 12 months from date of receipt, -20 to -70 °C as supplied.
- 1 month, 2 to 8 °C under sterile conditions after reconstitution.
- 6 months, -20 to -70 °C under sterile conditions after reconstitution.
Calculators
Background: ENPP-5
Long Name
Ectonucleotide Pyrophosphatase/Phosphodiesterase 5
Alternate Names
ENPP5, NPP5
Gene Symbol
ENPP5
UniProt
Additional ENPP-5 Products
Product Documents for Human ENPP‑5 Antibody
Product Specific Notices for Human ENPP‑5 Antibody
For research use only
Related Research Areas
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Protocols
Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.
- Cellular Response to Hypoxia Protocols
- Immunoprecipitation Protocol
- R&D Systems Quality Control Western Blot Protocol
- Troubleshooting Guide: Western Blot Figures
- Western Blot Conditions
- Western Blot Protocol
- Western Blot Protocol for Cell Lysates
- Western Blot Troubleshooting
- Western Blot Troubleshooting Guide
- View all Protocols, Troubleshooting, Illustrated assays and Webinars