Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunoprecipitation

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2A Clone # 500915
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Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human ENPP-5
Pro25-Ser430
Accession # Q9UJA9

Specificity

Detects human ENPP-5 in direct ELISAs and Western blots. In Western blots, no cross-reactivity with recombinant human (rh) ENPP-2, rhENPP-7, or recombinant mouse ENPP-7 is observed.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2A

Applications for Human ENPP‑5 Antibody

Application
Recommended Usage

Immunoprecipitation

25 µg/mL
Sample: Conditioned cell culture medium spiked with Recombinant Human ENPP-5, see our available Western blot detection antibodies

Western Blot

1 µg/mL
Sample: Recombinant Human ENPP-5

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.


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Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: ENPP-5

Ectonucleotide pyrophosphatase/phosphodiesterase 5 (ENPP-5) belongs to a group of nucleotide-metabolizing ecto-enzymes, which regulate the availability of extracellular nucleotides. It may also be involved in neuronal cell communication. The amino acid sequence of human ENPP-5 is 100%, 88%, and 82% identical to that of chimpanzee, dog and mouse/rat.

Long Name

Ectonucleotide Pyrophosphatase/Phosphodiesterase 5

Alternate Names

ENPP5, NPP5

Entrez Gene IDs

59084 (Human); 83965 (Mouse)

Gene Symbol

ENPP5

UniProt

Additional ENPP-5 Products

Product Documents for Human ENPP‑5 Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human ENPP‑5 Antibody

For research use only

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