Human FKBP12 Antibody (1049713)

R&D Systems | Catalog # MAB37771

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG2B Clone # 1049713
View all FKBP12 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived human FKBP12F36V protein
Gly1-Glu108
Accession # P62942

Specificity

Detects both Human FKBP12 and FKBP12F36V mutant in direct Elisas

Clonality

Monoclonal

Host

Mouse

Isotype

IgG2B

Scientific Data Images for Human FKBP12 Antibody (1049713)

FKBP12 in A431 human cell line.

FKBP12 was detected in immersion fixed A431 human epidermoid carcinoma cell line using Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) at 3 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; NL007) and counterstained with DAPI (blue). Specific staining was localized to cell cytoplasm. Staining was performed using our protocol for Fluorescent ICC Staining of Adherent Cells.

Detection of Human, Mouse, and Rat FKBP12 by Western Blot.

Western blot shows lysates of MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FKBP12 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Specificity of FKBP12 Shown by Western Blot.

Western blot shows recombinant human FKBP(F36V), FKBP12, and FKBP 12.6. PVDF membrane was probed with 1 µg/mL of Mouse Anti-Human FKBP12 Monoclonal Antibody (Catalog # MAB37771) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (HAF007). A specific band was detected for FKBP(F36V) and FKBP12 at approximately 12 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.

Applications for Human FKBP12 Antibody (1049713)

Application
Recommended Usage

Immunocytochemistry

3-25 µg/mL
Sample: Immersion fixed A431 human epidermoid carcinoma cell line

Western Blot

1 µg/mL
Sample: MCF-7 human breast cancer cell line, Neuro-2A mouse neuroblastoma cell line, and rat brain tissue

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Reconstitute at 0.5 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: FKBP12

FK506 binding protein, 12 kilodalton molecular weight (FKBP12), also called FKBP1, was originally characterized as a peptidyl-prolyl isomerase that catalyzes the transition between cis- and trans-proline residues critical for proper folding of proteins. The macrolide immunosuppressants FK506 (Tacrolimus) and rapamycin bind to FKBP12 with high affinity, while the structurally related compound cyclosporine binds with a much lower affinity (1). The binding of these drugs causes FKBP12 to become a potent inhibitor of calcineurin phosphatase activity (2) and TOR kinase activity (3). The inhibition of protein phosphatase activity is highly selective for calcineurin (2), making the FK506/FKBP12 complex a useful tool in the study of this enzyme. Knockout mice lacking FKBP12 are morphologically normal, but develop cardiomyopathies that may be related to dysregulation of ryanodyne receptors (4).

Long Name

12 kDa FK506 Binding Protein

Alternate Names

FKBP1a, PKC12, PPIASE

Entrez Gene IDs

2280 (Human); 14225 (Mouse); 25639 (Rat)

Gene Symbol

FKBP1A

UniProt

P62942

Additional FKBP12 Products

Product Documents for Human FKBP12 Antibody (1049713)

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human FKBP12 Antibody (1049713)

For research use only

Related Research Areas

Phosphatases and Regulators

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Protocols

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