Human FLRG Quantikine ELISA Kit

R&D Systems | Catalog # DFLRG0

R&D Systems
100% Guarantee

Key Product Details

Assay Length

4.5 hours

Sample Type & Volume Required Per Well

Cell Culture Supernates (100 µL), Serum (20 µL), EDTA Plasma (20 µL), Heparin Plasma (20 µL), Urine (50 µL), Human Milk (50 µL)

Sensitivity

8.55 pg/mL

Assay Range

62.5-4000 pg/mL
View all Follistatin-related Gene Protein/FLRG ELISA Kits »

Product Summary for Human FLRG Quantikine ELISA Kit

The Quantikine Human FLRG Immunoassay is a 4.5 hour solid-phase ELISA designed to measure human FLRG in cell culture supernates, serum, plasma, urine, and human milk. It contains NS0-expressed recombinant human FLRG and has been shown to accurately quantitate the recombinant factor. Results obtained using natural human FLRG showed linear curves that were parallel to the standard curves obtained using the Quantikine kit standards. These results indicate that this kit can be used to determine relative mass values for naturally occurring human FLRG.

Product Specifications

Assay Type

Solid Phase Sandwich ELISA

Format

96-well strip plate

Measurement

Quantitative ELISA

Detection Method

Colorimetric - 450nm (TMB)

Conjugate

HRP

Species

Human

Specificity

Natural and recombinant human FLRG

Cross-reactivity

< 0.5% cross-reactivity observed with available related molecules. < 50% cross-species reactivity observed with species tested.

Interference

Interference observed with 1 or more available related molecules.

精度

Intra-Assay Precision (Precision within an assay) Three samples of known concentration were tested on one plate to assess intra-assay precision.

Inter-Assay Precision (Precision between assays) Three samples of known concentration were tested in separate assays to assess inter-assay precision.

Cell Culture Supernates, Urine

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 468 1113 2252 494 1174 2331
Standard Deviation 10.4 23.0 55.2 32.9 69.0 143
CV% 2.2 2.1 2.5 6.7 5.9 6.1

EDTA Plasma, Heparin Plasma, Human Milk, Serum

Intra-Assay Precision Inter-Assay Precision
Sample 1 2 3 1 2 3
n 20 20 20 20 20 20
Mean (pg/mL) 448 1094 2306 486 1148 2282
Standard Deviation 15.4 18.5 49.5 29.5 53.0 122
CV% 3.4 1.7 2.1 6.1 4.6 5.3

Recovery for Human FLRG Quantikine ELISA Kit

The recovery of FLRG spiked to levels throughout the range of the assay was evaluated.

Sample Type Average % Recovery Range %
Cell Culture Media (n=4) 107 98-111
EDTA Plasma (n=4) 96 85-105
Heparin Plasma (n=4) 102 89-114
Serum (n=4) 100 91-112

Linearity

To assess the linearity of the assay, samples containing high concentrations of FLRG were serially diluted with the appropriate Calibrator Diluent to produce samples with values within the dynamic range of the assay.

Human Follistatin-related Gene Protein/FLRG ELISA Linearity

Scientific Data Images for Human FLRG Quantikine ELISA Kit

Human FLRG ELISA Serum/Plasma/Human Milk Standard Curve

Human FLRG ELISA Serum/Plasma/Human Milk Standard Curve

Human FLRG ELISA Cell Culture Supernate/Urine Standard Curve

Human FLRG ELISA Cell Culture Supernate/Urine Standard Curve

Preparation and Storage

Shipping

The product is shipped at ambient temperature. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Store the unopened product at 2 - 8 °C. Do not use past expiration date.

Background: Follistatin-related Gene Protein/FLRG

Follistatin-related gene protein (FLRG) has been shown to bind and inhibit the TGF-beta family ligands including activin, BMP-2, -6, -7, and GDF-8. FLRG is expressed most highly in heart, lung, kidney, and placenta, and is upregulated by TGF-beta and Activin via Smad proteins.

Alternate Names

Follistatin-like 3, FSTL3

Entrez Gene IDs

10272 (Human); 83554 (Mouse)

Gene Symbol

FSTL3

Additional Follistatin-related Gene Protein/FLRG Products

Product Documents for Human FLRG Quantikine ELISA Kit

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

Product Specific Notices for Human FLRG Quantikine ELISA Kit

For research use only

⚠ WARNING: This product can expose you to chemicals including N,N-Dimethylforamide, which is known to the State of California to cause cancer. For more information, go to www.P65Warnings.ca.gov.

Related Research Areas

Activin Family
BMP Family

Citations for Human FLRG Quantikine ELISA Kit

Customer Reviews for Human FLRG Quantikine ELISA Kit (1)

5 out of 5
1 Customer Rating
5 Stars
100%
4 Stars
0%
3 Stars
0%
2 Stars
0%
1 Stars
0%

Have you used Human FLRG Quantikine ELISA Kit?

Submit a review and receive an Amazon gift card!

$25/€18/£15/$25CAN/¥2500 Yen for a review with an image

$10/€7/£6/$10CAN/¥1110 Yen for a review without an image

Submit a review
Amazon Gift Card

Customer Images

Showing  1 - 1 的 1 review Showing All
Filter By:
Clear Filters X
  • Human FLRG Quantikine ELISA Kit
    Name: Anonymous
    Sample Tested: Serum
    Verified Customer | Posted 02/18/2021
    Human FLRG Quantikine ELISA Kit DFLRG0

There are no reviews that match your criteria.

Showing  1 - 1 的 1 review Showing All

Protocols

View specific protocols for Human FLRG Quantikine ELISA Kit (DFLRG0):

Refer to the product for complete assay procedure.

Bring all reagents and samples to room temperature before use. It is recommended that all samples, standards, and controls be assayed in duplicate.
  1.   Prepare all reagents, standard dilutions, and samples as directed in the product insert.
  2.   Remove excess microplate strips from the plate frame, return them to the foil pouch containing the desiccant pack, and reseal.
    3. 100 µL Assay Diluent
  3.   Add 100 µL of Assay Diluent to each well.
    4. 100 µL Standard, Control, or Sample
  4.   Add 100 µL of Standard, control, or sample to each well. Cover with a plate sealer, and incubate at room temperature for 2 hours on a horizontal orbital microplate shaker.
  5.   Aspirate each well and wash, repeating the process 3 times for a total of 4 washes.
    6. 200 µL Conjugate
  6.   Add 200 µL of Conjugate to each well. Cover with a new plate sealer, and incubate at room temperature for 2 hours on the shaker.
  7.   Aspirate and wash 4 times.
    8. 200 µL Substrate Solution
  8.   Add 200 µL Substrate Solution to each well. Incubate at room temperature for 30 minutes on the benchtop. PROTECT FROM LIGHT.
    9. 50 µL Stop Solution
  9.   Add 50 µL of Stop Solution to each well. Read at 450 nm within 30 minutes. Set wavelength correction to 540 nm or 570 nm.

Find general support by application which include: protocols, troubleshooting, illustrated assays, videos and webinars.

FAQs

No product specific FAQs exist for this product.

View all FAQs for ELISA Kits