Human GADD153 Alexa Fluor® 750-conjugated Antibody

Catalog #: FAB7224S Datasheet / COA / SDS

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FAB7224S-100UG

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All GADD153 Antibodies

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Summary Preparation and Storage Reconstitution Calculator Background Product Datasheets Related Research Areas

Human GADD153 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human GADD153 in direct ELISAs.

Source

Monoclonal Mouse IgG2b Clone # 671708

Purification

Protein A or G purified

Immunogen

E. coli-derived recombinant human GADD153
Met1-Ala169
Accession # P35638

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: GADD153

GADD153, also known as CCAAT-enhancer binding protein zeta (C/EBPz), DDIT, and CHOP, is a ubiquitous 29 kDa basic-leucine zipper transcription factor. GADD153 heterodimerization with other C/EBP factors inhibits their activity and alters their DNA sequence recognition. GADD153 inhibits Wnt signaling by dimerizing with the transcription factors TCF-3 and TCF-4. It is upregulated under conditions of cellular stress including DNA damage, oxidative damage, nutrient depletion, ER stress, and mechanical stress, whereupon it promotes the apoptotic response. The transcriptional activity of GADD153 is enhanced by phosphorylation at Ser 78 and Ser 81. Human GADD153 shares 88% and 90% amino acid sequence identity with mouse and rat GADD153, respectively.

Long Name

Growth Arrest and DNA Damage-inducible Protein GADD153

Entrez Gene IDs

1649 (Human); 13198 (Mouse); 29467 (Rat)

Alternate Names

C/EBP-homologous protein 10; C/EBP-homologous protein; CCAAT/enhancer-binding protein homologous protein; CEBP zeta; CHOP; CHOP10 CEBPZ; CHOP10; CHOP-10; CHOPC/EBP zeta; DDIT3; DDIT-3; DNA damage-inducible transcript 3 protein; DNA-damage-inducible transcript 3; GADD153; Growth arrest and DNA damage-inducible protein GADD153