Human Galectin-3BP/MAC-2BP Antibody

R&D Systems | Catalog # AF2226

R&D Systems

Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human, Mouse, Primate - Macaca mulatta (Rhesus Macaque)

Applications

Validated:

Western Blot, Immunocytochemistry, Simple Western

Cited:

Immunohistochemistry, Western Blot, Immunocytochemistry

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Galectin-3BP/MAC-2BP Primary Antibodies »

Product Specifications

Immunogen

Mouse myeloma cell line NS0-derived recombinant human Galectin‑3BP/MAC‑2BP
Val19-Asp585
Accession # Q08380

Specificity

Detects human Galectin‑3BP/MAC‑2BP in direct ELISAs and Western blots. In direct ELISAs, less than 20% cross-reactivity with recombinant mouse Galectin‑3BP/MAC‑2BP is observed.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Galectin-3BP/MAC-2BP Antibody

Detection of Human Galectin-3BP/MAC-2BP antibody by Western Blot.

Detection of Human Galectin‑3BP/MAC‑2BP by Western Blot.

Western blot shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line. PVDF membrane was probed with 1.0 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF019). A specific band was detected for Galectin-3BP/MAC-2BP at approximately 75 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Galectin-3BP/MAC-2BP antibody in HeLa Human Cell Line by Immunocytochemistry (ICC).

Galectin‑3BP/MAC‑2BP in HeLa Human Cell Line.

Galectin-3BP/MAC-2BP was detected in immersion fixed HeLa human cervical epithelial carcinoma cell line using Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) at 1.7 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red;NL001) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Galectin-3BP/MAC-2BP antibody by Simple WesternTM.

Detection of Human Galectin‑3BP/MAC‑2BP by Simple WesternTM.

Simple Western lane view shows lysates of HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line, loaded at 0.2 mg/mL. A specific band was detected for Galectin-3BP/MAC-2BP at approximately 115 kDa (as indicated) using 10 µg/mL of Goat Anti-Human Galectin-3BP/MAC-2BP Antigen Affinity-purified Polyclonal Antibody (Catalog # AF2226) followed by 1:50 dilution of HRP-conjugated Anti-Goat IgG Secondary Antibody (HAF109). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.
Detection of Galectin-3BP/MAC-2BP by Western Blot

Detection of Galectin-3BP/MAC-2BP by Western Blot

GAL3BP-induced inhibition of A beta production.a, the amounts of A beta 40 and A beta 42 in the conditioned media from H4-APPsw cells (Cont.) and H4-APPsw cells overexpressing GAL3BP (+GAL3BP) were measured using ELISAs. b, conditioned media were prepared from control HEK293 cells (Cont., lane 1), HEK293 cells with GAL3BP knockdown via RNAi (+RNAi, lane 2), and HEK293 cells with GAL3BP knockdown and GPLD1 overexpression (+RNAi, +GPLD1, lane 3) and subsequently added to H4-APPsw cells to test for their suppressive activity on A beta production. Western blot analysis confirmed the efficiency of RNAi for the amount of GAL3BP expression in the culture media (bottom). c, recombinant GAL3BP (5 μg/ml) prepared from HEK293 cells was added to H4-APPsw cell cultures, and the amounts of A beta 40 and A beta 42 in the media were determined. d, commercially available recombinant GAL3BP was added to H4-APPsw cell culture, and the amounts of A beta 40 and A beta 42 in the media were measured. a–d, the values in this graph are presented relative to the corresponding controls (n = 3, mean ± S.D. (error bars)). If error bars representing S.D. values are not visible, they are too small to be displayed. *, p < 0.05; **, p < 0.01; ***, p < 0.001 versus control by one-way ANOVA with Tukey's post-hoc test. Image collected and cropped by CiteAb from the following open publication (https://pubmed.ncbi.nlm.nih.gov/31996371), licensed under a CC-BY license. Not internally tested by R&D Systems.

Applications for Human Galectin-3BP/MAC-2BP Antibody

Application
Recommended Usage

Immunocytochemistry

1-15 µg/mL
Sample: Immersion fixed HeLa human cervical epithelial carcinoma cell line

Simple Western

10 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Western Blot

1 µg/mL
Sample: HeLa human cervical epithelial carcinoma cell line and HepG2 human hepatocellular carcinoma cell line

Reviewed Applications

Read 6 reviews rated 4 using AF2226 in the following applications:

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. See Certificate of Analysis for details.<br/>*Small pack size (-SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Galectin-3BP/MAC-2BP

Galectin-3 binding protein (Galectin-3BP), also known as MAC-2 binding protein (MAC-2BP or M2BP), and the 90 kDa tumor associated antigen (TAA90K or 90K), is a secreted glycoprotein of the scavenger receptor cysteine-rich (SRCR) superfamily (1, 2). Galectin-3BP binds Galectin-3 (formerly MAC-2) with high affinity, but also binds Galectins -1 and -7, several collagen types, fibronectin, beta 1 integrins and nidogen (3, 6, 7). It is widely expressed in all extracellular fluids and in pericellular areas of cell-rich tissues (1-3). The 585 amino acid (aa) human Galectin-3BP contains an 18 aa signal sequence and four definitive domains (4-6). Domain 1 is a group A scavenger receptor domain (4), domain 2 is a BTB/POZ domain that strongly mediates dimerization (5), and domain 3 is an IVR domain, that is also found following the POZ domain in Drosophila kelch protein. Although little is known about domain 4, recombinant domains 3 and 4 reproduce the solid-phase adhesion profile of full-length Galectin-3BP (5, 6). Glycosylation at seven N-linked sites, generates a molecular size of 85-97 kDa (1, 2, 6). Galectin-3BP dimers form linear and ring-shaped oligomers, most commonly decamers and dodecamers (3, 5). In vitro, Galectin-3BP has been shown to stimulate natural killer cells and lymphokine-activated killer cell activity (2). High Galectin-3BP expression has been correlated with tumor aggressiveness in several, but not all, study systems (7). Mature human Galectin-3BP shares 69% aa identity with mouse cyclophilin C-associated protein (CyCAP), which does not appear to bind Galectin-3 (8). Human Galectin-3BP also shares 73%, 67% and 68% aa identity with relatively uncharacterized orthologs in dog, rat and cow, respectively. A human N-terminally truncated sequence that begins within the BTB/POZ domain (aa 196) has been reported.

References

  1. Koths, K. et al. (1993) J. Biol. Chem. 268:14245.
  2. Ullrich, A. et al. (1994) J. Biol. Chem. 269:18401.
  3. Sasaki, T. et al. (1998) EMBO J. 17:1606.
  4. Hohenester, E. et al. (1999) Nat. Struct. Biol. 6:228.
  5. Muller, S. A. et al. (1999) J. Mol. Biol. 291:801.
  6. Hellstern, S. et al. (2002) J. Biol. Chem. 277:15690.
  7. Grassadonia, A. et al. (2004) Glycoconj. J. 19:551.
  8. Jalkanen, K. et al. (2001) Eur. J. Immunol. 31:3075.

Long Name

Galectin-3 Binding Protein

Alternate Names

90K, Galectin3BP, LGALS3BP, MAC-2BP

Entrez Gene IDs

3959 (Human)

Gene Symbol

LGALS3BP

UniProt

Q08380

Additional Galectin-3BP/MAC-2BP Products

Product Documents for Human Galectin-3BP/MAC-2BP Antibody

Certificate of Analysis

To download a Certificate of Analysis, please enter a lot or batch number in the search box below.

Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Galectin-3BP/MAC-2BP Antibody

For research use only

Citations for Human Galectin-3BP/MAC-2BP Antibody

Customer Reviews for Human Galectin-3BP/MAC-2BP Antibody (6)

4 out of 5
6 Customer Ratings
5 Stars
17%
4 Stars
67%
3 Stars
17%
2 Stars
0%
1 Stars
0%

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Showing  1 - 5 的 6 reviews Showing All
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  • Human Galectin-3BP/MAC-2BP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 01/14/2021
    Human Galectin-3BP/MAC-2BP Antibody AF2226
  • Human Galectin-3BP/MAC-2BP Antibody
    Name: Anonymous
    Application: ELISA
    Sample Tested: Plasma
    Species: Human
    Verified Customer | Posted 11/10/2018
  • Human Galectin-3BP/MAC-2BP Antibody
    Name: Anonymous
    Application: Microarrays
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 11/07/2018
  • Human Galectin-3BP/MAC-2BP Antibody
    Name: Anonymous
    Application: Microarray
    Sample Tested: EDTA Plasma
    Species: Human
    Verified Customer | Posted 10/31/2018
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 22496229
    Species: Human
    Verified Customer | Posted 01/06/2015
  • Name: Anonymous
    Application: Western Blot
    Sample Tested: See PMID 21182835
    Species: Human
    Verified Customer | Posted 01/06/2015

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