Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754)

R&D Systems | Catalog # MAB6490

R&D Systems

Key Product Details

Species Reactivity

Human

Applications

Western Blot, Immunocytochemistry, Simple Western

Label

Unconjugated

Antibody Source

Monoclonal Mouse IgG1 Clone # 856754
View all Glucuronosyltransferase 1A1/UGT1A1 Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Glucuronosyltransferase 1A1/UGT1A1
Leu60-Thr168
Accession # P22309

Specificity

Detects human Glucuronosyltransferase 1A1/UGT1A1 in ELISAs.

Clonality

Monoclonal

Host

Mouse

Isotype

IgG1

Scientific Data Images for Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754)

Detection of Human Glucuronosyltransferase 1A1/UGT1A1 antibody by Western Blot.

Detection of Human Glucuronosyltransferase 1A1/UGT1A1 by Western Blot.

Western blot shows lysates of COLO 205 human colorectal adenocarcinoma cell line. PVDF membrane was probed with 2 µg/mL of Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490) followed by HRP-conjugated Anti-Mouse IgG Secondary Antibody (Catalog # HAF018). A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 50 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 1.
Glucuronosyltransferase 1A1/UGT1A1 antibody in HepG2 Human Cell Line by Immunocytochemistry (ICC).

Glucuronosyltransferase 1A1/UGT1A1 in HepG2 Human Cell Line.

Glucuronosyltransferase 1A1/UGT1A1 was detected in immersion fixed HepG2 human hepatocellular carcinoma cell line using Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490) at 10 µg/mL for 3 hours at room temperature. Cells were stained using the NorthernLights™ 557-conjugated Anti-Mouse IgG Secondary Antibody (red; Catalog # NL007) and counterstained with DAPI (blue). Specific staining was localized to cytoplasm. View our protocol for Fluorescent ICC Staining of Cells on Coverslips.
Detection of Human Glucuronosyltransferase 1A1/UGT1A1 antibody by Simple WesternTM.

Detection of Human Glucuronosyltransferase 1A1/UGT1A1 by Simple WesternTM.

Simple Western lane view shows lysates of COLO 205 human colorectal adenocarcinoma cell line, loaded at 0.5 mg/mL. A specific band was detected for Glucuronosyltransferase 1A1/UGT1A1 at approximately 59 kDa (as indicated) using 20 µg/mL of Mouse Anti-Human Glucuronosyltransferase 1A1/UGT1A1 Monoclonal Antibody (Catalog # MAB6490). This experiment was conducted under reducing conditions and using the 12-230 kDa separation system.

Applications for Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754)

Application
Recommended Usage

Immunocytochemistry

8-25 µg/mL
Sample: Immersion fixed HepG2 human hepatocellular carcinoma cell line

Simple Western

20 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line

Western Blot

2 µg/mL
Sample: COLO 205 human colorectal adenocarcinoma cell line

Reviewed Applications

Read 1 review rated 3 using MAB6490 in the following applications:

Formulation, Preparation, and Storage

Purification

Protein A or G purified from hybridoma culture supernatant

Reconstitution

Sterile PBS to a final concentration of 0.5 mg/mL. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Glucuronosyltransferase 1A1/UGT1A1

UGT1A1 (UDP-glucuronosyltransferase 1-A1; also UDPGT 1-1 and HUG-BR1) is a 52-57 kDa member of the UGT1A subfamily, UGT family of enzymes. UGT1A1 is expressed by the liver, and catalyzes the conjugation of glucuronic acid (GA) from UDPGA to lipophilic acceptors such as (anti-cancer) drugs and bilirubin. Addition of glucuronic acid increases target solubility and facilitates elimination. Mature human UGT1A1 is a 508 amino acid (aa) type I transmembrane ER glycoprotein. It contains a 465 aa luminal domain (aa 26-490) plus a 26 aa cytoplasmic region. The luminal domain is unusual in that aa 157-176 are embedded in the ER membrane. Amino acids 29-444 contain the enzyme active site. The cytoplasmic tail appears to mediate noncovalent homodimerization, and heterodimerization with UGT2B. The signal sequence (aa 1-25) is normally cleaved, but a Lys15Arg mutation blocks insertion into the ER membrane. There are multiple point mutations that impact enzyme activity. One potential splice form is reported that shows a six aa substitution for aa 289-533. Over aa 60-186, human UGT1A1 shares 64% aa identity with mouse UGT1A1.

Long Name

UDP Glucuronosyltransferase 1A1

Alternate Names

GNT1, UDPGT 1-1, UG-BR1, UGT1, UGT1A

Entrez Gene IDs

54658 (Human); 394436 (Mouse); 24861 (Rat)

Gene Symbol

UGT1A1

UniProt

P22309 (Human)

Additional Glucuronosyltransferase 1A1/UGT1A1 Products

Product Documents for Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754)

Certificate of Analysis

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Product Specific Notices for Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754)

For research use only

Related Research Areas

Glycobiology-related Enzymes

Customer Reviews for Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754) (1)

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  • Human Glucuronosyltransferase 1A1/UGT1A1 Antibody
    Name: Sujay Ramanathan
    Application: Western Blot
    Sample Tested: Liver tissue
    Species: humanised mouse and Mouse
    Verified Customer | Posted 10/20/2017
    Human Glucuronosyltransferase 1A1/UGT1A1 Antibody (856754) MAB6490

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