Key Product Details

Species Reactivity

Validated:

Human

Cited:

Human

Applications

Validated:

Western Blot, Immunocytochemistry

Cited:

Western Blot

Label

Unconjugated

Antibody Source

Polyclonal Goat IgG
View all Hairless Primary Antibodies »

Product Specifications

Immunogen

E. coli-derived recombinant human Hairless
Met1-Thr277
Accession # O43593

Specificity

Detects human Hairless in direct ELISAs and Western blots.

Clonality

Polyclonal

Host

Goat

Isotype

IgG

Scientific Data Images for Human Hairless Antibody

Hairless antibody in NHEK Human Cell Line by Immunocytochemistry (ICC).

Hairless in NHEK Human Cell Line.

Hairless was detected in immersion fixed human NHEK epidermal keratinocytes using 10 µg/mL Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) for 3 hours at room temperature. Cells were stained with the NorthernLights™ 557-conjugated Anti-Goat IgG Secondary Antibody (red; Catalog # NL001) and counterstained with DAPI (blue). View our protocol for Fluorescent ICC Staining of Cells on Coverslips.

Detection of Human Hairless antibody by Western Blot.

Detection of Human Hairless by Western Blot.

Western blot shows lysates of HEK293 human embryonic kidney cell line. PVDF membrane was probed with 1 µg/mL of Goat Anti-Human Hairless Antigen Affinity-purified Polyclonal Antibody (Catalog # AF5708) followed by HRP-conjugated Anti-Goat IgG Secondary Antibody (Catalog # HAF019). A specific band was detected for Hairless at approximately 96 kDa (as indicated). This experiment was conducted under reducing conditions and using Immunoblot Buffer Group 8.

Applications for Human Hairless Antibody

Application
Recommended Usage

Immunocytochemistry

5-15 µg/mL
Sample: Immersion fixed human NHEK epidermal keratinocytes

Western Blot

1 µg/mL
Sample: HEK293 human embryonic kidney cell line

Formulation, Preparation, and Storage

Purification

Antigen Affinity-purified

Reconstitution

Reconstitute at 0.2 mg/mL in sterile PBS. For liquid material, refer to CoA for concentration.

Formulation

Lyophilized from a 0.2 μm filtered solution in PBS with Trehalose. *Small pack size (SP) is supplied either lyophilized or as a 0.2 µm filtered solution in PBS.

Shipping

Lyophilized product is shipped at ambient temperature. Liquid small pack size (-SP) is shipped with polar packs. Upon receipt, store immediately at the temperature recommended below.

Stability & Storage

Use a manual defrost freezer and avoid repeated freeze-thaw cycles.
  • 12 months from date of receipt, -20 to -70 °C as supplied.
  • 1 month, 2 to 8 °C under sterile conditions after reconstitution.
  • 6 months, -20 to -70 °C under sterile conditions after reconstitution.

Calculators

The reconstitution calculator allows you to quickly calculate the volume of a reagent to reconstitute your vial. Simply enter the mass of reagent and the target concentration and the calculator will determine the rest.

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Background: Hairless

Hairless (HR) is a predicted 145‑155 kDa protein that acts as a corepressor for multiple nuclear receptors. Although it was initially associated with atrichia (hairloss) and WISE gene suppression, it is now known to impact multiple genes in different cell types. In general, it binds both HDACs and select unligated nuclear receptors via multiple domains, thereby repressing gene transcription. Human HR is 1189 amino acids (aa) in length. It contains a C6-type zinc finger DNA binding region (aa 600‑625) and a Jmjc domain (aa 946‑1157) that blocks DNA methylation. The 1189 aa isoform is widely expressed, while a shorter isoform showing a deletion of aa 1072‑1126 is an HR isotype associated with hair follicle Keratin-14+ keratinocytes. An additional isoform shows a 25 aa substitution for aa 1091‑1189. Over
aa 1‑277, human HR shares 82% aa identity with mouse HR.

Alternate Names

ALUNC, AU, HR

Entrez Gene IDs

55806 (Human); 15460 (Mouse); 60563 (Rat)

Gene Symbol

HR

UniProt

O43593

Additional Hairless Products

Product Documents for Human Hairless Antibody

Certificate of Analysis

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Note: Certificate of Analysis not available for kit components.

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Product Specific Notices for Human Hairless Antibody

For research use only

Citations for Human Hairless Antibody

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Protocols

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