Human HOXA1 Alexa Fluor® 750-conjugated Antibody

Catalog #: AF5014S Datasheet / COA / SDS

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AF5014S-100UG

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Human HOXA1 Alexa Fluor® 750-conjugated Antibody Summary

Species Reactivity

Human

Specificity

Detects human HOXA1 in direct ELISAs and Western blots. In direct ELISAs and Western blots, less than 5% cross-reactivity with recombinant human (rh) HOXB1, rhHOXB4, and rhHOXA9 is observed.

Source

Polyclonal Goat IgG

Purification

Antigen Affinity-purified

Immunogen

E. coli-derived recombinant human HOXA1
Met1-Asp118
Accession # P49639

Formulation

Supplied 0.2mg/ml in 1X PBS with RDF1 and 0.09% Sodium Azide

Label

Alexa Fluor 750 (Excitation= 749 nm, Emission= 775 nm)

Applications

Recommended Concentration

Sample

Western Blot

Optimal dilution of this antibody should be experimentally determined.

Immunocytochemistry

Optimal dilution of this antibody should be experimentally determined.

Please Note: Optimal dilutions should be determined by each laboratory for each application. General Protocols are available in the Technical Information section on our website.

Reconstitution Calculator

Preparation and Storage

Shipping

The product is shipped with polar packs. Upon receipt, store it immediately at the temperature recommended below.

Stability & Storage

Protect from light. Do not freeze. 12 months from date of receipt, 2 to 8 °C as supplied

Background: HOXA1

HOXA1 (Homeobox A1; also Hox-1F) is a 35 kDa member of the Antp homeobox family of transcription factors. It is expressed in epithelium and is upregulated by E‑cadherin signaling, whereupon it suppresses apoptosis and promotes anchorage dependent growth. Human HOXA1 is 335 amino acids (aa) in length. It contains two poly-His sequences (aa 65-74 and 142-146), a PBX binding segment (aa 204-209) and a DNA binding homeobox domain (aa 229-288). There are multiple splice variants. There is a potential alternate start site at Met6 that may be paired with a nine aa substitution for the C‑terminal 117 aa, or a 19 aa substitution for the C‑terminal 217 amino acids. Over aa 1-118, human HOXA1 shares 92% and 90% aa identity with mouse and canine HOXA1, respectively.

Long Name

Homeobox A1

Entrez Gene IDs

3198 (Human); 15394 (Mouse); 25607 (Rat)

Alternate Names

BSAS; homeo box A1; homeobox A1; Homeobox protein Hox-1F; homeobox protein Hox-A1; Hox 1.6-like protein; HOX A1 homeodomain protein; HOX1; HOX1F; HOX1Fhomeobox 1F; HOXA1; lab-like protein; MGC45232